2002 Fiscal Year Final Research Report Summary
Development of a Gene Transfer and Functional Evaluation System for an Isolated Single Cardiac Myocyte as a model for the Gene Therapy
| Project/Area Number |
13670692
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SUGIURA Seiryo The University of Tokyo, Graduate School of Frontier Sciences, Professor, 大学院・新領域創成科学研究科, 教授 (10272551)
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| Co-Investigator(Kenkyū-buntansha) |
SATA Masataka The University of Tokyo, Graduate School of Medicine, Assistant Professor, 医学部附属病院, 助手 (80345214)
YAMASHITA Hiroshi The University of Tokyo, Graduate School of Medicine, Assistant Professor, 医学部附属病院, 助手 (50323572)
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| Project Period (FY) |
2001 – 2002
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| Keywords | cardiac myocyte / contractile force / carbon fiber |
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| Research Abstract |
In order to study the mechanical activity of a single cardiac myocyte under a wide range of load, we have developed a novel force measurement system using carbon fibers. Newly fabricated Carbon fibers greatly facilitate the firm attachment of cell membrane to the fibers. A pair of fibers was attached to both ends of the cell ; the rigid fiber as a mechanical ground and the compliant fiber for the strain gauge. By connecting the compliant fiber to the piezoelectric translator and applying the position signal to the driver, we could make the myocyte contract under wide range of loading conditions. Furthermore, we have implemented the optical system for the measurement of intracellular calcium concentration thus the simultaneous recording of force and calcium transient of a single cardiac myocyte was made possible. We observed a strong load dependence of calcium transient, which shows clear contrast to the previous reports. Feedback control of the system also enabled us to study the function of a single cardiac myocyte under physiological loading sequence. We have also measured the force developed by a myocyte transfected with GFP adenovirus. This system can be a useful tool in studying the mechanical activity of the cardiac myocyte under genetic interventions thus serving as a useful tool in the study of heart failure.
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Research Products
(8 results)
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[Publications] Yasuda S, Sugiura S, Kobayakawa N, Fujita H, Yamashita H, Katoh K, Saeki Y, Kaneko H, Suda Y, Nagai R, Sugi H: "A novel method to study contraction characteristics of a single cardiac myocyte using carbon fibers coupled with a feedback system"Am J Physiol. 281. H144-H1446 (2001)
Description
「研究成果報告書概要(和文)」より
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[Publications] Yasuda S, Sugiura S, Kobayakawa N, Fujita H, Yamashita H, Katoh K, Saeki Y, Kaneko H, Suda Y, Nagai R, and Sugi H: "A novel method to study contraction characteristics of a single cardiac myocyte using carbon fibers coupled with a feedback system"Am J Physiol. 281. H1442-1446 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Sata M, Nishimatsu H, Suzuki E, Sugiura S, Yoshizumi M, Ouchi Y, Hirata Y, Nagai R, Masataka Sata, Hiroaki Nishimatsu, Etsu Suzuki, Seiryo Sugiura, Masao Yoshizumi, Yasuyoshi Ouchi, Yasunobu Hirata, Ryozo Nagai: "Endothelial nitric oxide synthase is essential for the HMG-CoA reductase inhibitor cerivastatin to promote collateral growth in response to ischemia"FASEBJ. 15. U20-U33 (2001)
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「研究成果報告書概要(欧文)」より
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