2002 Fiscal Year Final Research Report Summary
Stem Cell Transplantation Attenuates Deleterious Ventricular Remodeling and Improves Cardiac Performance After Myocardial Infarction
| Project/Area Number |
13670738
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Circulatory organs internal medicine
|
|---|
| Research Institution | Osaka City University |
|---|
Principal Investigator |
TAKEUCHI Kazuhide Osaka City University, Graduate School of Medicine, Professor, 大学院・医学研究科, 教授 (80117952)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YOSHIYAMA Minoru Osaka City University, Graduate School of Medicine, Associate Professor, 大学院・医学研究科, 助教授 (30240956)
YOSHIKAWA Junichi Osaka City University, Graduate School of Medicine, Professor, 大学院・医学研究科, 教授 (60275245)
|
|---|
| Project Period (FY) |
2001 – 2002
|
| Keywords | Myocardial infarction / Remodeling / Cell therapy / Stem Cell / Heart failure / Signal transduction |
|---|
| Research Abstract |
Myocardial infarction (MI) promotes deleterious remodeling of the myocardium, resulting in ventricular dilation and pump dysfunction. We examined whether supplementing infarcted myocardium with stem cell would attenuate deleterious remodeling, and enhance contractile performance. Experimental MI was induced by 24-hours coronary ligation followed by reperfusion in adult male Lewis rats and neonatal myocytes were injected directly into the infarct region. Three groups of animals were studied at 4 weeks after cell therapy: noninfarcted control (control), MI plus sham injection (MI), and MI plus stem cell injection (MI+cell). In vivo cardiac function was assessed by echocardiography. MI and MI+cell hearts had indistinguishable infarct sizes of 30% of the LV. At 4 weeks after cell therapy, 92% (13 of 14) of MI+cell hearts showed evidence of myocytes graft survival. MI+cell hearts exhibited attenuation of global ventricular dilation and reduced septum and free wall diameter compared with MI hearts not receiving cell therapy. Implanted stem cell form viable grafts in infarcted myocardium, resulting in enhanced contractile function and attenuated ventricular dilation. These data illustrate that stem cell implantation after MI improves both in systolic and diastolic dysfunction and deleterious remodeling and suggests that cellular implantation may be beneficial after MI.
|
Research Products
(13 results)
-
-
-
-
-
-
-
-
-
-
[Publications] Ehara S, Ueda M, Naruko T, Haze K, Matsuo T, Ogami M, Ikura Y, Itabe H, Komatsu R, Yoshiyama M, Takeuchi K, Yoshikawa J.: "Pathophysiological role of oxidized low-density lipoprotein in plaque instability in coronary artery diseases."J Diabetes Complications.. 16. 60-64 (2002)
Description
「研究成果報告書概要(欧文)」より
-
-
-
