2004 Fiscal Year Final Research Report Summary
Analysis of Expression of LAT1 in Childhood Leukemia and Effect of Inhibitors on Proliferation Inhibition
| Project/Area Number |
13670836
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Kyorin University |
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Principal Investigator |
BESSHO Fumio KYORIN UNIVERSITY, DEPARTMENT of PEDIATRICS, PROFESSOR, 医学部, 教授 (40010285)
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| Co-Investigator(Kenkyū-buntansha) |
ENDOU Hitoshi KYORIN UNIVERSITY, DEPARTMENT of PHARMACOLOGY, PROFESSOR, 医学部, 教授 (20101115)
KANAI Katsuyoshi KYORIN UNIVERSITY, DEPARTMENT of PHARMACOLOGY, PROFESSOR, 医学部, 教授 (60204533)
YAN Kunimasa KYORIN UNIVERSITY, DEPARTMENT of PEDIATRICS, ASSOCIATE PROFESSOR, 医学部, 助教授 (70255389)
YOSHINO Hiroshi KYORIN UNIVERSITY, DEPARTMENT of PEDIATRICS, ASSISTANT, 医学部, 助手 (30333032)
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| Project Period (FY) |
2001 – 2004
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| Keywords | LAT1 / BCH / inhibitor / bone marrow cells / acute lymphocytic leukemia / acute myeloid leukemia / cell lines / hematopoietic stem cells |
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| Research Abstract |
Expression of LAT1 was investigated for childhood leukemia cell lines and bone marrow cells from children with various kinds of leukemia at the diagnosis and during remission by Western blotting method, fluorescence-labeled antibody method and RT-PCR.
Effect of BCH, one of inhibitors of LAT1, on cell liability was also investigated using MTT assay.
Expression of LAT1 was found for all cell lines and bone marrow cells at diagnosis with all kinds of employed methods. Difference of expression of LAT1 was not observed among investigate leukemic cell lines with various biological natures and bone marrow leukemic cells with different prognoses and different cell types. Expression of LAT1 was also observed some of bone marrow cells during the remission phase.
BCH could reduce liability of leukemic cell lines by dose-dependent manner. However, rate of reduction of liability decreased during increasing dose and LC_<50> could not be obtained by the BCH concentration of above 300 microM.
Above result indicates necessity of stronger inhibitors to treat leukemia. Moreover, LAT1 was expressed on bone marrow cells during the remission. Since LAT1 is known to express on peripheral leukocytes and bone marrow, proliferation of normal bone marrow cells might be inhibited with LAT1 inhibitor. Other inhibitors with higher selectivity may be able to decrease liability more efficiently. Since currently available inhibitors with such characteristics are water insoluble, new inhibitors should be searched. Effects of inhibitors on hematopoietic stem cells are also needed to be tested in further studies.
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Research Products
(12 results)
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[Journal Article] Expression and Functional characterization of the system L amino acid transporter in KB human oral epidermoid carcinoma cells.2004
Author(s)
Yoon J.H., Kim Y.B., Kim M.S., Park J.C., Kook J.K., Jung H.M., Kim S.G., Yoo H., Ko Y.M., Lee S.H., Kim B.Y., Chun H.S., Kanai Y., Endou H., Kim do K.
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Journal Title
Cancer Lett 205
Pages: 215-226
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] System L-amino acid transporters are Differently expressed in rat astrocyte and C6 glioma cells.2004
Author(s)
Kim do K., Kim I.J., Hwang S., Kook J.H., Lee M.C., Shin B.A., Bae C.S., Yoon J.H., Ahn S.G., Kim S.A., Kanai Y., Endou H., Kim J.K.
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Journal Title
Neurosci Res 50
Pages: 437-446
Description
「研究成果報告書概要(欧文)」より
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