2002 Fiscal Year Final Research Report Summary
Cytokines and TIMPs in skin tumors
| Project/Area Number |
13670868
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KIKUCHI Kanako The University of Tokyo, Faculty of Medicine, Associate Professor, 医学部附属病院, 助教授 (60186195)
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| Project Period (FY) |
2001 – 2002
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| Keywords | Melanoma / metallo proteinase / adhesion molecule / TIMP / Wound healing |
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| Research Abstract |
We investigated the role of tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-2 in the growth regulation. Primary (PM-WK and KHm-4), recurrent primary (RPM-EP and RPM-MC), lymph node metastatic (MM-LH) melanoma cell lines were used. Reverse transcriptase-coupled polymerase-chain reaction and Western blotting revealed that all expressed and produced TIMP-1 and TIMP-2 except for PM-WK, which neither expressed nor produced TIMP-1. We found that the TIMP-1 production level was correlated with the cell migration rate. Moreover, TIMP-1 enhanced the cell migration of PM-WK. The growth of the primary melanoma cell lines was stimulated by TIMP-1 and inhibited by TIMP-2. In contrast, the growth of the visceral metastatic melanoma cell line was stimulated by TIMP-2.
We found K14 expression may be a marker of tumor progression in Bowen's disease, which is in situ malignancy of the epidermis.
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