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2002 Fiscal Year Final Research Report Summary

Role of ADAM family in normal human epidermal keratinocytes

Research Project

Project/Area Number 13670890
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionEhime University

Principal Investigator

SHIRAKATA Yuji  Ehime University, Faculty of Medicine, Instructor, 医学部, 助手 (50226320)

Project Period (FY) 2001 – 2002
KeywordsADAM / mRNA / adenovirus vector / dominant negative / real time PCR / keratinocyte / psoriasis vulgaris
Research Abstract

ADAM family is transtmembrane protein that shares a disintegrin domain and metalloproteinase domain. To date, more than 30 members have been identified, although their role and regulation in normal human keratinocytes have not been fully determined. In order to assess whether ADAM family play an important role for keratinocyte proliferation and differentiation, we investigated their expression in normal human keratinocytes. We constructed Taqman probe for 19 ADAM family members. Total RNA was harvested from normal human keratinocytes under serum free condition, and then the expression level was determined by real time polymerase chain reaction. Keratinocyte express 15 ADAM family members at proliferating phase, and ADAM9 was upregulated by the addition of EGF. To further characterize the involvement of ADAM in skin disease, we investigated ADAM expression in lesional epidermis of psoriasis vulgaris, a hyperproliferative disorder. Epidermis was separated by heat activation from 13 skin samples from psoriasis vulgaris and 11 skin samples from normal healthy skin, and total RNA was extracted. ADAM expression was determined by real time PCR. There is no difference about ADAM12, 21, TS3, TS4, TS5 and TS6 expression between psoriasis vulgaris and normal health skin, whereas ADAM10, 17, 20 and 28 were downregulated in psoriatic epidermis, and ADAM30 was upregulated in psoriatic epidermis. ADAM15 was dramatically downregulated in psoriatic epidermis. Furthermore, ADAM19 and ADAMTS7 were significantly upregulated in psoriatic epidermis. Taking together, we demonstrated that normal human keratinocytes express more than 15 ADAM families, and ADAM family might be involved in the pathogenesis of psoriasis vulgaris.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Tohyama M, Shirakara Y, Yamasaki K, Sayama K, Hashimoto K: "Differentiated keratinocytes are responsible for TNF-alpha regulated production of macrophage inflammatory protein 3alpha/CCL20, a potent chemokine for Langerhans cells"Journal of Dermatological Science. 27(2). 130-1392 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sayama K, Hanakawa Y, Shirakata Y, Yamasaki K, Sawada Y, Sun L, Yamanishi K, Ichijo H, Hashimoto K: "Apoptosis signal regulating kinase 1 (ASK1) is an intracellular inducer of keratinocyte differentiation"Journal of Biological Chemistry. 276. 999-1004 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sayama K, Yamasaki K, Hanakawa Y, Shirakata Y, Tokumaru S, Ijuin T, Takenawa T, Hashimoto K: "Phosphatidyl inositol 3 kinase is a key regulator of early phase differentiation in keratinocytes"Journal of Biological Chemistry. 277. 40390-40396 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hanakawa Y, Amagai M, Shirakata Y, Yahata Y, Tokumaru S, Yamasaki K, Tohyama M, Sayama K, Hashimoto K: "Differentiatl effects of desmoglein 1 and desmoglein 3 on desmosome formation"Journal of Investigative Dermatology. 119. 1231-1236 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kobayashi M, Amagai M, Kuroda-Kinoshita K, Hashimoto T, Shirakata Y, Hashimoto K, Nishikawa T: "BP180 ELISA using bacterial recombinant NC16a protein as a diagnostic and monitoring tool for bullous pemphigoid"Journal of Dermatological Science. 30. 224-232 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsuda T, Thoyama M, Yamasaki K, Shirakata Y, Yahata Y, Tokumaru S, Sayama K, Hashimoto K: "Lack of evidence for TARC/CCL17 production by normal human keratinocytes in vitro"Journal of Dermatological Science. (in press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Wada T, Shirakata Y, Takahashi H, Murakami S, Iizuka H, Suzuki H, Hashimoto K: "A Japanese Case of Segmental Darier's Disease Caused by Mosaicism for the ATP2A2 Mutation"British Journal of Dermatology. (In press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tohyama M, Shirakara Y, Yamasaki K, Sayama K, Hashimoto K: "Differentiated keratinocytes are responsible for TMF-alpha regulated production of macrophage inflammatory protein 3alpha/CCL20, a potent chemokine for Langerhans cells"Journal of Dermatological Science. 27 (2). 130-139 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sayama K, Hanakawa Y, Shirakata Y, Yamasaki K, Sawada Y, Sun L, Yamanishi K, Ichijo H, Hashimoto K: "Apoptosis signal regulating kinase 1 (ASK1) is an intracellular inducer of keratinocyte differentiation"Journal of Biological Chemistry. 276. 999-1004 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sayama K, Yamasaki K, Hanakawa Y, Shirakata Y, Tokumaru S, Ijuin T, Takenawa T, Hashimoto K: "Phosphatidyl inositol 3 kinase is a key regulator of early phase differentiation in keratinocytes"Journal of Biological Chemistry. 277. 40390-40396 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hanakawa Y, Amagai M, Shirakata Y, Yahata Y, Tokumaru S, Yamasaki K, Tohyama M, Sayama K, Hashimoto K: "Differentiatl effects of desmoglein 1 and desmoglein 3 on desmosome formation"Journal of Invest Dermatol.. 119. 1231-1236 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kobayashi M, Amagai M, Kuroda-Kinoshita K, Hashimoto T, Shirakata Y, Hashimoto K, Nishikawa T: "BP180 ELISA using bacterial recombinant NC16a protein as a diagnostic and monitoring tool for bullous pemphigoid"Journal of Dermatol Sci.. 30. 224-232 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsuda T, Thoyama M, Yamasaki K, Shirakata Y, Yahata Y, Tokumaru S, Sayama K, Hashimoto K: "Lack of evidence for TARC/CCL17 production by normal human keratinocytes in vitro"Journal of Dermatol Sci.. in press.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Wada T, Shirakata Y, Takahashi H, Murakami S, Iizuka H, Suzuki H, Hashimoto K: "A Japanese Case of Segmental Darier's Disease Caused by Mosaicism for the ATP2A2 Mutation"British Journal of Dermatol.. in press.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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