• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2003 Fiscal Year Final Research Report Summary

Fundamental Study on objective diagnosis and therapy effect decision of the dementia by the molecular imaging.

Research Project

Project/Area Number 13670925
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Radiation science
Research InstitutionKanazawa University

Principal Investigator

MORI Hirofumi  Kanazawa University, Advanced Science Research Center, Professor, 学際科学実験センター, 教授 (90019604)

Co-Investigator(Kenkyū-buntansha) KINUYA Seigo  Kanazawa University, Graduate School of Medical Sciences, Assistant, 医学系研究科, 助手 (20281024)
SHIBA Kazuhiro  Kanazawa University, Advanced Science Research Center, Associate Professor, 学際科学実験センター, 助教授 (40143929)
Project Period (FY) 2001 – 2003
KeywordsDementia / Receptor / Transporter / Neurotransplantation / Radiopharmaceutical
Research Abstract

Parkinsonian rats were stereotaxically infused 6-OHDA into the unilateral mesostriatal dopamine pathway and measured apomorphine-induced rotational behavior. Controls were infused saline instead of neurotoxin. Animals were divided into transplantation(TP) and sham-operated(S) groups. Ipsilateral vagal nodosal ganglion was surgically removed horn die neck and stereotaxically transplanted as nerve fragments into the ipsilateral striatum 2 weeks after nerotoxin infusion Sequential brain sections underwent autoradiography of dopaminergic system 2 weeks after operation. Densities of dopamine D1,D2 receptors and dopamine transporter(DAT) were assessed by in-vitro autoradiography using[^3H]SCH23390,[^3H]YM-09151-2 and[^3H]GBR12935. Significant DAT decrease in striatum was shown in the model animals compared with controls(P<0.0001). D_2 receptor in the ipsilateral striatum increased significantly in transplanted animals compared with sham-operated animals(S/R-,0.97±0.08;TP/R-,1.08±0.08;p=0.039 … More and S/R+,1.06±0.06;TP/R+,1.19±0.16;p=0.023), whereas DAT and D_1 receptor showed no significant changes. Our results suggest that vagal autotransplantation induces upregulation of D_2 receptor in the ipsilateral striatum. Autotransplantation showed no significant effect on DAT, although DAT imaging is a sensitive test for dopaminergic damage. We need hurther study on dopaminergic system imaging after neural transplantation.
We evaluated the potencies of radioiodimted(-)-o-iodovesamicol [(-)-oIV] as a selective vesicular acetylcholine tmnsporter(VAChT) mapping agen. (-)-[^<125>1] oIV exhibited significant accumulationin rat brain. The regional brain distribution of radioactivity was similar for both (-)-[^<125>1] oIV and [^3H]vesamicol. The accumulation of (-)-[^<125>1] oIV in the brain was significant reduced by post-administration of unlabeled vesamicol(0.5μmol/kg^<-1>) and(-)-[^<125>1] oIV μmol/kg-1] in the other hand, the post-administration of sigma ligands hardy affected the accumulation of (-)-[^<125>1] oIV in die brain. These studies showed that (-)-[^<125>1] oIV, as well as [^3H]vesamicol, bound to VAChT with high affinity in die rat brain. Funhemore, (-)-[^<125>1] oIV binding in the ipsilateral cortex to the lesion was significantly reduced by 17.0%, compared with that in the contralateral cortex in a unilateral NBM-lesioned rat. These results suggested that radioiodimted(-)-[^<125>1] oIV may potentially be useful for the diagnosis of cholinergic neurodegenerative disorders. Less

  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Shiba, K, Mori, H., et al.: "Chracterization of (-)radioisodinated (-)-ortho-iodovesamicol binding in rat braid preparations"Life Sciences. 71. 1591-1598 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shiba, K, Mori, H., et al.: "Evaluation of radioiodinated(-)-o-iodovesamicol as a radiotracer for mapping the vesicular acetylcholine transporter"Annals of Nuclear Medicine. 17・6. 451-456 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shiba K.: "Chracterization of (-)radioisodinated (-)-ortho-iodovesamicol binding in rat brain preparations"Life Sciences. 71. 1591-1598 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shiba K.: "Evaluation of radioiodinated(-)-o-iodovesamicol as a radiotracer for mapping the vesicular acetylcholine transporter"Annals of Nuclear Medicine. 17(6). 451-456 (2003)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2005-04-19  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi