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2002 Fiscal Year Final Research Report Summary

Study on nitric oxide and GABA in the hypothalamic paraventricular nucleus and prefrontal cortex under stress

Research Project

Project/Area Number 13671018
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Psychiatric science
Research Institution宮崎医科大学

Principal Investigator

ISHIZUKA Yuta  Miyazaki Medical College, Lecturer, 医学部, 講師 (20264377)

Co-Investigator(Kenkyū-buntansha) ISHIDA Yasushi  Miyazaki Medical College, Professor, 医学部, 教授 (20212897)
HASHIGUCHI Hiroyuki  Miyazaki Medical College, Assistant, 医学部, 助手 (40305090)
Project Period (FY) 2001 – 2002
Keywordsparaventricular nucleus / medial prefrontal cortex / interleukin-1β / nitric oxide / γ-aminobutyric acid(GABA)
Research Abstract

We have suggested previously that intraperitoneal (I.p.) administration of interleukin-1β (IL-1β) increases nitric oxide (NO) levels in the hypothalamic paraventricular nucleus (PVN). Using an in vivo brain microdialysis technique, we measured extracellular levels of NO metabolites (Nox^-) in the hypothalamic PVN upon perfusion of selective γ-aminobutyric acid (GABA) receptor antagonist as well as agonist into the medial prefrontal cortex (mPFC) through a microdialysis probe. We examined the effects of selective GABAA receptor antagonist and agonist on IL-1β-induced NO releases in the PVN in conscious rats. Double immunostaining for Fos and neuronal nitric oxide synthase (nNOS) was also used to examine whether nNOS-immunoreactive neurons in the PVN are activated to express Fos immunoreactivity by I.p. injection of IL-Iβ in the rat. The elevated levels of Nox^- in the PVN after I.p. administration IL-1β (4μg/kg) were attenuated by perfusion of muscimol (50 μM), a GABAA receptor agonist, into the mPFC. We also examined the effect of GABAB receptor antagonist and agonist on IL-1β-induced NO releases in the PVN in conscious rats. Perfusion of baclofen (250 μM), a GABAB receptor agonist, into the mPFC had no effect on the elevated levels of Nox^- in the PVN after I.p administration IL-1β(4 μg/kg). Perfusion of saclofen (50 μM), a GABAB receptor antagonist, into the mPFC had no effect on the elevated levels of Nox^- in the PVN after I.p. administration IL-1β(4 μg/kg). Quantitative analysis revealed that some nNOS-positive PVN neurons are activated by IL-1β(4 μg/kg, I.p.) administration. These findings are likely to provide helpful clues to our understanding of the inhibitory modulation of IL-1β-induced NO metabolites releases in the PVN by GABAA and GABAB receptors in the mPFC.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Jin Q-H, Ueda Y, Ishizuka Y, Kannan H: "Cardiovascular changes induced by central hypertonic saline are accompanied by glutamate release in awake rats"Am J Physiol Regul Integr Comp Physiol. 281. R1224-R1231 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishida Y, Hashiguchi H, Takeda R, Ishizuka Y, Mitsuyama Y, Kannan H: "Conditioned-fear stress increases Fos expression in monoaminergic and GABAergic neurons of the locus coeruleus and dorsal raphe nuclei"Synapse. 45. 46-51 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jin Q-H, Kunitake T, Chu C-P, Qiu D-L, Kato K, Ishizuka Y, Kannan H: "Possible involvement of nitric oxide in the central salt-loading-induced cardiovascular responses in conscious rats"Brain Res. 963. 224-231 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kannan H, Kunitake T, Kato K, Saita M, Shirasaka T, Nose K, Jin Q-H, Ishizuka Y: "Adaptive changes in the sympathetic nervous and endocrine systems during fever induced by interleukin-1β in conscious rats : Potential neural mechanism"Kosaka M, et al.(eds) : Thermotherapy for Neoplasia, Inflammation and Pain Springer-Verlag, Tokyo. 280-289 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jin Q.-H., Ueda Y., Ishizuka Y., Kunitake T. & Kannan H.: "Cardiovascular changes induced by central hypertonic saline are accompanied by glutamate release in awake rats."Am J Physiol Regul Integr Comp Physiol.. 281. R1224-R1231 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishida Y., Hashiguchi H., Takeda R., Ishizuka Y., Mitsuyama Y., Kannan H., Nishimori T. & Nakahara D.: "Conditioned-fear stress increases Fos expression in monoaminergic and GABAergic neurons of the locus coeruleus and dorsal raphe nuclei."Synapse. 45. 46-51 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jin Q.-H., Kunitake T., Chu C.-P., Qiu D.-L., Kato K., Ishizuka Y. & Kannan H.: "Possible involvement of nitric oxide in the central salt-loading-induced cardiovascular responses in conscious rats."Brain Res.. 963. 224-231 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kannan H., Kunitake T., Kato K., Saita M., Shirasaka T., Nose K., Jin Q.-H. & Ishizuka Y., Ed. Kosaka M et al.: "Adaptive changes in the sympathetic nervous and endocrine systems during fever induced by interleukin-1β in conscious rats : Potential neural mechanism., Thermotherapy for Neoplasia, Inflammation, and Pain."Springer-Verlag. 280-289 (2001)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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