2002 Fiscal Year Final Research Report Summary
Molecular biological approach to investigate higher brain functions
| Project/Area Number |
13671027
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Psychiatric science
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| Research Institution | Tokyo Metropolitan University of Health Sciences (2002)
Jichi Medical University (2001) |
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Principal Investigator |
SUDA Haruhiko Tokyo Metropolitan University of Health Sciences Professor, 保健科学部, 教授 (40051784)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIJIMA Koichi Jichi Medical School Associate Professor, 精神医学, 助教授 (30198460)
NIBUYA Masashi National Defense Medical College Lecturer, 精神科学, 講師 (00228256)
TATEYAMA Masato Tokyo Metropolitan University of Health Sciences Professor, 保健科学部, 教授 (60118966)
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| Project Period (FY) |
2001 – 2002
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| Keywords | post-transcriptional regulation / GIRK2 / AU-rich element / RNA binding protein / cloning / brain / rat / Down syndrome |
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| Research Abstract |
GIRK2 (G protein-gated inwardly rectifying K(+) channel 2) located on the Down syndrome region 21q22.2 in humans has been reported to have several alternative transcripts and transcripts longer than 4 kb that do not have the poly-A tail. We sequenced GIRK2 transcripts with a long 3'-untranslated region (3'-UTR) containing multiple adenylate uridylate-rich elements (AREs) with the poly-A tail. In a 16-kb transcript, 28 AUUUA pentanucleotides, 9 AUUUUA hexanucleotides, 5 AUUUUUA heptanucleotides, and 3 UUAUUUA[U/A] [U/A] nonanucleotides were found. Northern blot and in situ hybridization revealed abundant expression of the 16-kb transcripts in the rat brain despite no detectable signals in other tissues examined. The AREs have been reported to mediate the turnover of mRNAs encoding proteins regulating cellular proliferation/differentiation and body response to inflammatory and environmental stimuli. This is the first study indicating that ion channel transcripts have multiple AREs.
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