2003 Fiscal Year Final Research Report Summary
Research on HPA axis and clock genes.
| Project/Area Number |
13671029
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Psychiatric science
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| Research Institution | Saitama Medical University |
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Principal Investigator |
IKEDA Masaaki SAITAMA MEDICAL SCHOOL, MEDICINE, ASSISTANT PROFESSOR, 医学部, 講師 (80232198)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIMURA Kazunori SAITAMA MEDICAL SHOOL, MEDICINE, ASSISTANT PROFESSOR, 医学部, 助手 (20158497)
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| Project Period (FY) |
2001 – 2003
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| Keywords | clock gene / HPA axis / circadian rhythm / BMAL1 / transcription factor / transcriptional regulation / promoter / orphan nuclear receptor |
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| Research Abstract |
In mammals, the biological clock is controlled from the suprachiasmatic nucleus (SCN), an area of the brain in the hypothalamus. Light sets the circadian rhythm via the retino-hypothalamic tract (RHT) to a 24-hour light-dark cycle. Much of the transcription of output genes is mediated by BMAL1 : CLOCK acting on E-boxes in their promoter regions, indicating the importance of the level of transcription of the Bmal1 and Clock genes. To investigate the transcriptional control of Bmal1 genes, we used a luciferase reporter assay using Bmal1 promoter constructs to find factors that regulate Bmal1 transcription. This revealed that the reporter activity of the Bmal1 promoter constructs was enhanced by RORα, RORβ, and RORγ, and was inhibited by Rev-Erbβ via ROR-response elements(ROR-RE). RORα was the most potent activator of Bmal1 transcription. Therefore, we postulate that members of the ROR and Rev-Erb family expressed in the SCN bind to the Bmal1 promoter via ROR-RE, and that the level of Bmal1 activation caused by the members of the ROR and Rev-Erb family is determined mainly by the sum of their recruitment to the binding sites.
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[Publications] Iwase T, Kajimura N, Uchiyama M, Ebisawa T, Yoshimura K, Kamei Y, Shibui K, Kim K, Kudo Y, Katoh M, Watanabe T, Nakajima T, Ozeki Y, Sugishita M, Hori T, Ikeda M, Toyoshima R, Inoue Y, Yamada N, Mishima K, Nomura M, Ozaki N, Okawa M, Takahashi K, Yamauchi T.: "Mutation screening of the human Clock gene in circadian rhythm sleep disorders"Psychiatry Res. 09. 121-128 (2002)
Description
「研究成果報告書概要(和文)」より
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[Publications] Ikeda M, Honma S, Abe H, Namihira M, Yu W, Nomura M, Honma K: "Zeitgebers, entrainment and masking of the circadian system (Honma K, and Honma S Eds)"Hokkaido University Press, Sapporo, Japan. 5 (2001)
Description
「研究成果報告書概要(和文)」より
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[Publications] Iwase T, Kajimura N, Uchiyama M, Ebisawa T, Yoshimura K, Kamei Y, Shibui K, Kim K, Kudo Y, Katoh M, Watanabe T, Nakajima T, Ozeki Y, Sugishita M, Hori T, Ikeda_M, Toyoshima R, Inoue Y, Yamada N, Mishima K, Nomura M, Ozaki N, Okawa M, Takahashi K, Yamauchi T: "Mutation screening of the human Clock gene in circadian rhythm sleep disorders"Psychiatry Res. 09. 121-128 (2002)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Ikeda M, Honma S, Abe H, Namihira M, Yu W, Nomura M, Honma K: "Molecular cloning and characterization of the clock gene Bmal1"Zeitgebers, entrainment and masking of the circadian system (Honma K, and Honma S Eds)(Hokkaido University Press, Sapporo, Japan). 101-105 (2001)
Description
「研究成果報告書概要(欧文)」より
