2002 Fiscal Year Final Research Report Summary
Possible mechanism of hematopietic stem cells homing to the bone marrow microenvironment
| Project/Area Number |
13671048
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | KYOTO UNIVERSITY (2002)
The University of Tokyo (2001) |
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Principal Investigator |
MAEKAWA Taira Departments of Transfusion Medicine and Cell Therapy, Professor of Medicine, 医学研究科, 教授 (80229286)
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| Co-Investigator(Kenkyū-buntansha) |
TSUJI Kouichiro Department of Pediatrics, The Institute of Medical Science, University of Tokyo, Associate Professor, 医科学研究所, 助教授 (50179991)
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| Project Period (FY) |
2001 – 2002
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| Keywords | cord blood transplantation / hematopietic stem cell / chemokine / homing |
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| Research Abstract |
We found that the stromal cell derived factor-1/pre-B cell growth stimulating factor (SDF1/PBSF) receptor, CXCR4, is expressed on human CD34^+ bone marrow (BM) cells. Stringently FACS-sorted CD34^+CXCR4^+ BM cells completely lack myeloid, erythroid, megakaryocytic, and mixed colony forming potential (myeloid progenitors), but give rise to B and T lymphoid progenitors, whereas CD34^+CXCR4^- BM cells can generate colonies formed by myeloid progenitors and can also develop into these lymphoid progenitors. Therefore, expression of CXCR4 on CD34^+ BM cells can allow lymphoid progenitors to be discriminated from myeloid progenitors. Since CD34^+CXCR4^+ cells are differentiated from CD34^+CXCR4^- cells, multipotential progenitors located in the BM are likely to be negative for CXCR4 expression. CXCR4 seems to be expressed earlier than the interleukin-7 receptor and terminal deoxynucleotidyl transferase during early lymphohemopoiesis. These results suggest that the expression of CXCR4 on CD34^+ BM cells is one of the phenotypic alterations for committed lymphoid progenitors.
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[Publications] Ma, R., Wada, M., Yoshino, H., Ebihara, Y., Ishii, T., Manabe, A., Tanaka, R., Maekawa. T., Itoh, M., Mugishima, H., Asano, S., Nakahata, T., Tsuji, K.: "Development of human lymphohematopoietic stem/progenitor cells defined by CD34 and CD81 expressions"Blood. 97. 3755-3762 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Tomonari, A., Tojo, A., Iseki, T., Ooi, J., Nagayama, H., Ogami, K., Maekawa, T., Shirafuji, N., Tani, K., Asano, S.: "Severe autoimmune thrombocytopenia after allogeneic bone marrow transplantation for aplastic anemia"Int J Hematol. 74(2). 228-232 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Kimura, S., Horie, A., Hiki, Y., Yamamoto, C., Suzuki, S., Kuroda, J., Kobayashi, Y., Yoshikawa, T., Deguchi, M., Mineo, T., Kato, G., Karasuno, T., Hiraoka, A., Maekawa. T.: "Nephrotic syndrome with crescent formation and massive IgA deposition and hypothyroidism following hematopoietic stem cell transplantation"Blood. 101. 4219-4221 (2003)
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「研究成果報告書概要(欧文)」より
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[Publications] Kuroda, J., Kimura S., Kobayashi, Y., Yoshikawa, T., Urasaki, Y., Ueda, T., Enjo, F., Tokuda, H., Ottmann, O.G., Maekawa, T.: "The third-generation bisphosphonate Zoledronate synergistically augments the anti-Ph+ leukemia activity of imatinib mesylate"Blood. (in press). (2003)
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「研究成果報告書概要(欧文)」より
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[Publications] Yoshimasu, T., Manabe, A., Ebihara, Y., Tanaka, R., Ooi, J., Iseki T., Shirafuji N., Maekawa, T., Asano S., Yoshikawa N., Tsuji K.: "MxA expression in patients with viral infection after allogeneic stem cell transplantation"Bone Marrow Transplant. (in press). (2003)
Description
「研究成果報告書概要(欧文)」より
