2002 Fiscal Year Final Research Report Summary
Regulation of diuresis by 2 types of antidiuretic hormone receptor in chronic renal failure and therapeutic investigation of edema
| Project/Area Number |
13671121
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | Kumamoto University |
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Principal Investigator |
NONOGUCHI Hiroshi Kumamoto University, Department of Nephrology, Associate Professor, 医学薬学研究科・腎臓内科, 助教授 (30218341)
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| Co-Investigator(Kenkyū-buntansha) |
TOMITA Kimio Kumamoto University, Department of Nephrology, Professor, 医学薬学研究科・腎臓内科, 教授 (40114772)
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| Project Period (FY) |
2001 – 2002
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| Keywords | antidiuretic hormone / V1a receptor / V2 receptor / aquaporin 2 / trafficking / metabolic acidosis / 転写活性 |
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| Research Abstract |
We first investigated urinary excretion of aquaporin 2 (AQP2) using Western blot analysis. However, AQP2 was not detectable in the urine. We have been still trying to detect urinary excretion of AQP2 by increasing the sensitivity of Western blot analysis. Urinary excretion of AQP2 was also examined in rats with metabolic acidosis. Urinary excretion of AQP2 was significantly decreased in metabolic acidosis. However, the expressions of AQP2 mRNA and protein in outer medullary collecting ducts were increased. Differential centrifugation revealed that AQP2 expression was decreased in membrane-rich fraction but increased in intracellular vesicle-rich fraction. These data suggest that trafficking of AQP2 from intracellular vesicles to apical membrane is inhibited in metabolic acidosis. The precise mechanisms of the disturbance has to be examined further.
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[Publications] Nonoguchi H., Kiyama S., Inoue H., Nakayama Y., Inoue T., Kohda Y., Machida K., Tajima A., Kitamura K., Miyoshi T., Shimada H., Shimada H., Tajiri M., Honda Y., Tanaka M., Tomita K.: "Angiotensin-converting enzyme inhibitor withdrawal and ACE gene polymorphism"Clin Nephrol. (in press).
Description
「研究成果報告書概要(欧文)」より
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[Publications] Narikiyo T, Kitamura K, Adachi M, Miyoshi T, Iwashita K, Shiraishi N, Nonoguchi H, Chen LM, Chai KX, Chao J, Tomita K.: "Regulation of prostasin by aldosterone in the kidney"J. Clin. Invest. 109. 401-408 (2002)
Description
「研究成果報告書概要(欧文)」より
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