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2002 Fiscal Year Final Research Report Summary

Cell Biology of the Pancreatic Stem Cells

Research Project

Project/Area Number 13671176
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Metabolomics
Research InstitutionInstitute for Molecular and Cellular Regulation, Gunma University

Principal Investigator

KUBOHARA Yuzuru  Institute for Molecular and Cellular Regulation, Associate Professor, 生体調節研究所, 助教授 (00221937)

Co-Investigator(Kenkyū-buntansha) MOGAMI Hideo  Hamamatsu University School of Medicine, Associate Professor, 医学部, 助教授 (90311604)
Project Period (FY) 2001 – 2002
Keywordsregeneration medicine / insulin / pancreatic stem cell / βcell / differentiation / diabetes / pancreas / activin
Research Abstract

The final goal of our study is to establish a new therapeutic approach for treatment of type II diabetes. In the present study, we investigate to elucidate the characteristics of the pancreatic stem cells and regulatory mechanism for the regulation of growth and differentiation of these cells. We first studied the changes in the expression of transcription factors during the differentiation of AR42J, a model of the pancreatic stem cells, during differentiation to insulin-producing cells. We found that activinA induced differentiation to endocrine cells and neurogenin3 is a critical transcription factor. We then studied the changes in the expression of activin A in the pancreatic duct after reduction of the beta cell mas. We found that the expression of activin A was induced after the reduction of the beta cell mass. Given that activin A is a critical differentiation factor for the pancreatic stem cells, the induction of acivin A in the pancreatic duct may be a crucial step for the initiation of the beta cell neogenesis. Finally, we extablished a culture of the pancreatic ductal cells and found that these cells differentiate into insulin-secreting cells after the treatment with a combanation of activn A and betacellulin. We are currently studying the properties of differentiated cells.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Zhang, Y.Q., Mashima, H., Kojima, I.: "Changes in the expression of transcription factors in AR42J cells during differentiation to insulin-secreting cells"Diabetes. 50. S10-S14 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujimaki, S., Kubohara, Y., Kobayashi, I., Kojima, I.: "Caspase-independent apoptosis induced by DIF in lNS-I cells"Eur. J. Pharmacol.. 421. 93-100 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Zhang, Y.Q., Zhang, H., Maeshima, A., Miyagawa, I., Kojima, I.: "Up-regulation of the expression of activins in the pancreatic duct by reduction of the β cell mass"Endocrinology. 143. 3540-3547 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Zhang, Y.Q., Maeshima, H. and Kojima, I.: "Changes in the expression of transcription factors in AR42J cells during differentiation to insulin-secreting cells"Diabetes. 50. S10-S14 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujimaki, S., Kubohara, Y., Kobayashi, I. and Kojima, I.: "Caspase-independent apoptosis induced by DIF in INS-1 cells"Eur. J. Pharmacol.. 421. 93-100 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Zhang, Y.Q., Zhang, H., Maeshima, A., Miyagawa, I. and Kojima, I.: "Up-regulation of the expression of activins in the pancreatic duct by reduction of the β cell mass"Endocrinology. 143. 3540-3547 (2002)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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