2002 Fiscal Year Final Research Report Summary
The mechanism of glucose-induced time-dependent potentiation of insulin secretion from pancreatic β cells and its alteration in diabetic state
| Project/Area Number |
13671186
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Metabolomics
|
|---|
| Research Institution | KYOTO UNIVERSITY |
|---|
Principal Investigator |
FUJIMOTO Shimpei Kyoto University, Graduate School of Medicine, Instructor, 医学研究科, 助手 (00333576)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
NAGASHIMA Kazuaki Kyoto University, Graduate School of Medicine, Instructor, 医学研究科, 助手 (40324628)
|
|---|
| Project Period (FY) |
2001 – 2002
|
| Keywords | pancreatic islet / pancreatic β cell / insulin secretion / glucose / priming effect / exocytotic system / intracellular Ca^<2+> concentration / intracelluar ATP concentration |
|---|
| Research Abstract |
A brief exposure to elevated glucose augments the insulin secretory response of islets to subsequent stimulation. The site of this time-dependent potentiation (priming effect) of glucose in the mechanism of the regulation of insulin secretion is not completely known, however. Insulin release triggered by a depolarizing concentration of K^+ in the presence of basal glucose is markedly enhanced in primed rat islets. To clarify the role of priming on Ca^<2+> and ATP efficacy in the exocytotic apparatus, islets were electrically permeabilized to vary the intracellular Ca^<2+> and ATP concentration according to the extracellular medium, and insulin release was evaluated. Ca^<2+ >and ATP efficacy in Ca^<2+> and ATP-dependent insulin secretion was not affected by priming, and alteration of the intracellular Ca^<2+> concentration after depolarization cannot account for the phenomenon. There was no difference in ATP content before depolarization between nonprimed and primed islets. Moreover, the decline in ATP level after depolarization with basal glucose was observed in both primed and nonprimed islets. However, a reduced decline in ATP level in the early phase was observed in primed islets. In addition, oligomycin, a mitochondrial metabolism inhibitor, abolished the difference in ATP level between primed and nonprimed islets, suggesting that mitochondrial ATP production may be linked to the phenomenon
|
Research Products
(12 results)
