2002 Fiscal Year Final Research Report Summary
DEVELOPMENT OF ELISA METHOD FOR THE MEASUREMENT OF APOLIPOPROTEIN B48
| Project/Area Number |
13671191
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | OSAKA UNIVERSITY |
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Principal Investigator |
HIRANO Ken-ichi Osaka University Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (30332737)
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| Co-Investigator(Kenkyū-buntansha) |
YAMASHITA Shizuya Osaka University Graduate School of Medicine, Associate Professor, 医学系研究科, 助教授 (60243242)
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| Project Period (FY) |
2001 – 2002
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| Keywords | Apolipoprotein B48 / ELISA / Hyperlipidemia / CD36 / Chylomicron / Chylomicron remnant |
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| Research Abstract |
We have developed the ELISA method for the measurement of serum apolipoprotein (apo) B-48, which is expressed exclusively in the intestines in humans. The novel method indicates that the serum levels of apo B48 were increased with patients with type I, III, V hyperlipidemia. In addition, it has been found that CD36 deficiency, which is relatively frequent in the Japanese population and associated with insulin resistance, had higher levels of apo B48 after the oral fat loading test. These data indicated that the above disorders may have the impaired metabolism of small intestine-derived lipids. The increased levels of apo B48 might be related to the development of atherosclerotic cardiovascular disease in patients with the above dysllpidemia and hyperlipidemia.
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