2003 Fiscal Year Final Research Report Summary
Biochyperhemical evaluation of isolated porcine liver system perfused with oxygenated blood by ECMO
| Project/Area Number |
13671262
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Kanazawa Medical University |
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Principal Investigator |
IKAWA Hiromiti Kanazawa Medical University, Professor, 医学部, 教授 (20124935)
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| Co-Investigator(Kenkyū-buntansha) |
MASUYAMA Hiroaki Kanazawa Medical University, Assistant, 医学部, 助手 (90255480)
OKAMOTO Sinya Kanazawa Medical University, Assistant Professor, 医学部, 講師 (00329377)
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| Project Period (FY) |
2001 – 2003
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| Keywords | Liver transplantation / perfusion preservation / hyperglyicemia |
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| Research Abstract |
Aim : The optimal condition of liver perfusion was investigated in order to prolong the preservation time of liver graft and also to avoid primary non-function or re-perfusion injury.
Materials and Methods : Twenty one porcine livers were purfused with oxygenated blood by ECMO, using venous-venous bypass via portal vein to infrahepatic IVC. After supra IVC and hepatic artery were ligated, thliver was harvested and kept on perfusing in 37℃ lactate Ringer solution.
Results : Since some amount of bile juice was excreted throughout the experiment, biological activity of the liver grafts seem to be maintained essentially by meaning of perfusion with oxygenated blood for more than 12 hours. However, it was made clear that the oxygenated perfusion via portal vein be responsible for severe metabolic and biochemical problems. They were hyponatremia, metabolic asidosis and hyperglycemia which were resistant to insulin administration.
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Research Products
(6 results)
