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2002 Fiscal Year Final Research Report Summary

Invasion mechanism of Pancreatic cells : the relationship Glial cell line-derived neurotrophic factor and integrin

Research Project

Project/Area Number 13671325
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionNagoya City University

Principal Investigator

TAKEYAMA Hiromitsu  Nagoya City University, Graduate School of Medical Sciences, Associate Professor, 大学院・医学研究科, 助教授 (00216946)

Co-Investigator(Kenkyū-buntansha) SAWAI Hirozumi  Nagoya City University, Graduate School of Medical Sciences, Rsearch Associate, 大学院・医学研究科, 助手 (40336681)
SATO Mikinori  Nagoya City University, Graduate School of Medical Sciences, Research Associate, 大学院・医学研究科, 助手 (20305551)
MANABE Tadao  Nagoya City University, Graduate School of Medical Sciences, Professor, 大学院・医学研究科, 教授 (80127141)
Project Period (FY) 2001 – 2002
KeywordsPancreatic / neural invasion / GDNF / RET / GFRalpha-1 / integrin
Research Abstract

Prineural invasion is a prominent clinical feature of pancreatic cancer. The invasion system forms by multiple steps, but it has not cleared yet. We pay attention to the integrins and investigated its expression and alteration by GDNF. In this study, we use four human pancreatic cancer cell lines which were all found to express the receptor RET and GFRalpha-1 for GDNF. In the invasion assay, all human pancreatic cancer cell lines stimulated by GDNF increased the invasive ability for ECMs. In the adhesion assay, they increased the adhesive ability for ECMs. All pancreatic cancer cell lines stimulated by GDNF, their adhesive and the invasive ability in inhibited by anti-RET antibody, anti-GFRalpha-1 antibody and anti-betal antibody. In the flow-cytometric analysis, the integrin subunit alpha2, alpha3, alpha5, alpha6 and betal expressed in the all pancreatic carcinoma cell lines. In the cellular enzyme-linked immunosorbent assay, the integrin subunit expressed strongly under stimulating by GDNF. In the all pancreatic cancer cell lines, the expression of integrin subunit, the invasive ability and the adhesive ability appeare to increase under stimulating by GDNF. NF-kappaB was enhanced in the all pancreatic tumor cells stimulated by GDNF. We conclude that GDNF and integrin have very important role for the neural' invasive system of pancreatic cancer cells. We suggest that the control of the receptor or integrin makes new therapy.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] HITOSHI FUNAHASHI: "Alteration of Integrin Expression by Grial Cell Line-Derived Neurotrophic Factor (GDNF) in Human Pancreatic Cancer Cells"Pancreas. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] TADAO MANABE: "Current aspects of the pathophysiology of acute pancreatitis and therapeutic effects of an inflammatory cell infiltration inhibitor"Journal of Gastroenterology. 38. 305-307 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] HIROZUMI SAWAI: "Tumor malignancy and gene expression analysis"Surgery Frontier. 10. 33-38 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] HITOSHI FUNAHASHI, HIROMITSU TAKEYAMA, HIROZUMI SAWAI, AKIYOSHI FURUTA, MIKINORI SATO, YUJI OKADA, TESUSHI HAYAKAWA, MORITSUGU TANAKA, and TADAO MANABE: "Alteration of Integrin Expression by Grial Cell Line-Derived Neurotrophic Factor (GDNF) in Human Pancreatic Cancer Cells"Pancreas. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] TADAO MANABE: "Current aspects of the pathophysiology of acute pancreatitis and therapeutic effects of an inflammatory cell infiltration inhibitor"Journal of Gastroenterology. 38(3). 305-307 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] HIRDZUMI SAWAI, HITOSHI FUNAHASHI, and TADAO MANABE: "Tumor malignancy and gene expression analysis"Surgery Frontier. 10(1). 33-38 (2003)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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