2002 Fiscal Year Final Research Report Summary
Prevention of hepatic ischemia-reperfusion injury by gene transfer of IкB antisense and its application to liver transplantation
| Project/Area Number |
13671342
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Keio University |
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Principal Investigator |
SHIMAZU Motohide Keio University, School of Medicine, Assistant Professor, 医学部, 講師 (70124948)
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| Co-Investigator(Kenkyū-buntansha) |
SHINODA Masahiro Keio University, School of Medicine, Assistant, 医学部, 助手 (50286499)
TAKAYANAGI Atsushi Keio University, School of Medicine, Assistant, 医学部, 助手 (80245464)
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| Project Period (FY) |
2001 – 2002
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| Keywords | IкB / NFкB / antisence / genetransfer / ischemia-reperfusion injury / liver transplantation |
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| Research Abstract |
NFкB is a transcriptional factor which plays a major role in production of inflammatory cytokines and regulation of apoptosis. However, it is still unclear whether up-regulation of NFкB has a cytoprotective effect or not in hepatic ischemia-reperfusion injury. We already constructed a recombinant adenovirus vector expressing a truncated form of the inhibition protein IкB (Adex IкB), and demonstrated that transfer of Adex IкB inhibited NFкB activation and aggravated hepatic ischemia-reperfusion injury in rats. In the present study, we tried to construct Adex IкB antisense suppressing IкB production and investigated the effect of IкB antisense gene transfer to NFкB activation and ischemia-reperfusion injury. In vitro, amount of IкB was not decreased in cultured cells infected Adex IкB antisense, although IкB antisense cDNA was detected by RT-PCR. In hepatic ischemia-reperfusion model in rats, administration of Adex IкB antisense did not attenuate hepatic injury compared to the control group
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[Publications] Morisue,A, Wakabayashi,G, Shimazu,M, Mukai,M, Matsumoto,K, Kawachi,S, Yoshida,M, Yamamoto,S, Kitajima,M: "The role of Nitric Oxide after a short period of liver ischemia-reperfusion"J Surg Res. 109. 101-109 (2003)
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「研究成果報告書概要(欧文)」より
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[Publications] Shinoda,M, Shimazu,M, Wakabayashi,G, Tanabe,M, Hoshino,K, Kitajima,M: "Tumor necrosis factor suppression and microcirculatory disturbance amelioration in ischemia/reperfusion injury of rat liver after ischemic preconditioning"J Gastroenterol Hepatol. 17. 1211-1219 (2002)
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[Publications] Harada,H, Wakabayashi,G, Takayanagi,A, Shimazu,M, Matsumoto,K, Obara,H, Shimizu,N, Kitajima,M: "Transfer of the Interleukin-1 receptor antagonist gene into rat liver abrogates hepatic ischemia-reperfusion injury"Transplantation. 74. 1434-1441 (2002)
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「研究成果報告書概要(欧文)」より
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[Publications] Shinoda,M, Shimazu,M, Matsuda,S, Wakabayashi,G, Tanabe,M, Hoshino,K, Kamei,S, Koyasu,S, Kitajima,M: "c-Jun N-terminal kinase activation during warm hepatic ischemia/reperfusion injuries in a rat model"Wound Repair Regen. 10. 314-319 (2002)
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「研究成果報告書概要(欧文)」より
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[Publications] Yamamoto,S, Tanabe,M, Wakabayashi,G, Shimazu,M, Matsumoto,K, Kitahjima,M: "The Role of Tumor Ncrosis Factor-α and Interleukin- 1β in Ischemia-Reperfusion Injury of the Rat Small Intestine. Journal of Surgical Research, 99 : 134-141, 2001"J Surg Res. 99. 134-141 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Kato,Y, Shimazu,M, Walabayashi,G, Tanabe,M, Morikawa,Y, Hoshino,K, Harada,H, Kadomura,T, Obara,H, Urakami,H, Shinoda,M, Kitajima,M: "Significance of Portal Venous Flow in Graft Regeneration After Living Liver Transplantation"Transplant Proc. 33. 1484-1485 (2001)
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「研究成果報告書概要(欧文)」より
