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2002 Fiscal Year Final Research Report Summary

Experimental study for reconstruction of portal vein and inferior vena cava in liver surgery

Research Project

Project/Area Number 13671343
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionDokkyo University School of Medicine (2002)
Showa University (2001)

Principal Investigator

YAMAGUCHI Masahiko  Dokkyo University School of Medicine, Professor, 医学部, 教授 (00266149)

Co-Investigator(Kenkyū-buntansha) NAKANO Hiroshi  Showa University, School of Medicine, Assistant Professor, 医学部, 講師 (10241035)
Project Period (FY) 2001 – 2002
Keywordsreconstruction of IVC / hepatectomy / hepatocyte growth factor / ecdothelial seeding
Research Abstract

Results in 2001: Adenovirus vector containing hepatocyte growth factor cDNA (AdHGF) was propagated in 293 cells and concectrated with purification. Vascular endothelial cells (ECs) were isolated from canine jugular vein and cultured in M199+10%FCS. Cytotoxicity and HGF production were investigated to determine the most effective dose of AdHGF. As a result, it was concluded that 50 PFU of AdHGF per cell were the most effective dose.
Results in 2003: Replacement of IVC with PTFE graft where AdHGF-transfected ECs were seeded was performed using 5 dogs after right hepatectomy. Two out of 5 dogs were survived, but three other dogs were dead within a week after the operation. Causes of death included untolerable damages and bleeding by the operation. Survived two dogs were sacrificed 4 weeks after the operation to investigate the liver regeneration and seeds grafts. There was no clot adherent to the graft where viable ECs were attached. Liver regeneration of hepatectomized dogs with seeded IVC graft was not different from hepatectomized dogs with unseeded graft. Serum concentrations of HGF were measured in the operation. However, levels of HGF in all serum samples were less than measurable ones. It is suggested that HGF expression levels from AdHGF-transfected ECs on the graft might not be enough to show biological activity.

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Shimabukuro K, Yamaguchi M, et al.: "Hepatocyte growth factor/scatter factor is implicated in the mode"Gynecologic Oncology. 83. 205-215 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Yamaguchi, H.Nemoto, et al.: "Effects of Insulin-like growth factor-1 on short bowel syndrome"Eur Serg Res. 33. 291-296 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okuyama T, Oya M, Yamaguchi Ml: "Budding(sprouting)as a useful prognostic marker in colorectal mucinous carcinoma"Jpn J Clin Oncol. 32. 412-416 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masahiko Yamaguchi, Genshu Tate, Satoshi Wakasugi, et al.: "Diffuse cystic malformation with early cancer of the stomach"Showa Univ Med Sc. 14. 75-81 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masahiko Yamaguchi, Genshu Tate, Yasuo Yoshizawa, et al.: "Hepatocellular Carcinoma in a Patient with Acromegaly and High Serum Levels of Insulin-like Growth Factor I : Report of a Case"Surg Today. 32. 1008-1011 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Min Li, Genshu Tate, Masahiko Yamaguchi, et al.: "Hepatocellular Carcinoma in a Patient with Acromegaly and High Serum Levels of Insulin-like Growth Factor I : Report of a Case"Showa Univ J Med Sci. 14. 169-173 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山口真彦, 熊田馨: "今日の消化器疾患治療指針(第2版):Budd-Chiari症候群"多賀須幸男、三田村圭二、幕内雅敏編集、医学書院. 930 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimabukuro K, Yamaguchi M, et al.: "Hepatocyte growth factor/scatter is implicated in the mode---"Gynecologic Oncology. 83. 205-215 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M. Yamaguchi, H. Nemoto, et al.: "Effects of Insulin-like growth factor-1 on short bowel syndrome---"Eur Serg Res. 33. 291-296 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okuyama T, Oya M, Yamaguchi M: "Budding (sprouting) as a useful prognostic marker in colorectal mucinous carcinoma"Jpn Clin Oncol. 32. 412-416 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masahiko Yamaguchi, Genshu Tate, Satoshi Wakasugi, et al.: "Diffuse cystic malformation with early cancer of the stomach"Showa Univ Med Sc. 14. 75 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masahiko Yamaguchi, Genshu Tate, Yasuo Yoshizawa, et al.: "Hepatocellular Carcinoma in a Patient with Acromegaly and High Serum Levels of Insulin-like Growth Factor I: Report of a Case"Surg Today. 32. 1008-1011 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Min Li, Genshu Tate, Masahiko Yamaguchi, et al.: "Hepatocellular Carcinoma in a Patient with Acromegaly and High Serum Levels of Insulin-like Growth Factor I: Report of a Case"Showa Univ J Med Sci. 14. 169-173 (2002)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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