Role of protein kinase C and chimaerin in esophageal cancer cells

2003 Fiscal Year Final Research Report Summary

• Project/Area Number: 13671361
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Kurume University
• Principal Investigator: FUJII Teruhiko KURUME UNIVERSITY, SCHOOL OF MEDICINE, ASSISTANT PROFESSOR, 医学部, 講師 (50199288)
• Co-Investigator(Kenkyū-buntansha): YAMANA Hideaki KURUME UNIVERSITY, SCHOOL OF MEDICINE, PROFESSOR, 医学部, 教授 (30140669) ; SUEYOSHI Susumu KURUME UNIVERSITY, SCHOOL OF MEDICINE, ASSISTANT PROFESSOR, 医学部, 講師 (30235840)
• Project Period (FY): 2001 – 2003
• Keywords: esophageal cancer / protein kinase C / b2 chimaerin

Research Abstract

PKC and β2chimaerin play important role in intracellular signaling, however, the role of PKC and β2chimaerin are not clear in esophageal cancer cells. The aim of this study was to explore the role of PKCδ and β2chimaerin as a mediator of phorbor ester-mediated responses in KE3 esophageal cancer cells. The phorbor ester PMA induces G_2 cell cycle arrest in KE3 esophageal cancer cells. A significant inhibition in cell number was observed upon infection with PKCδAdV, however, β2chimaerin overexpression did not cause any significant changes in cell number or cell cycle ditribution. PKCδ is closely involved in PMA-induced inhibition in KE3 cell growth. The results. of the experiments with ChimAdV exclude the possibility of β2chimaerin involvement. However, β2chimaerin plays important role in intracellular signaling, further study is required.

Research Products

• [Publications] Garcia-Bermejo ML et al.: "DAG-lactones, a new class of PKC agonists, induce apoptosis in LNCaP prostate cancer cells by selective activation of PKC α"J Biol Chem. 277. 645-655 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Fujii T. et al.: "Climicopathologic study of revascularization and VEGF expression in superficial esophageal carcinoma"Int J Oncol. 21. 1181-1187 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Nakagawa S. et al.: "Cell growth inhibition by all-trans retinoic acid in SKBR-3 breast cancer cells"Mol Carcinogen. 38. 106-116 (2003)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Fujii T. et al.: "Immunohistological study of cell cycle-related factor, oncogene expression, cell proliferation in adenocarchom a of Barrett's esophagus"Oncol Rep. 10. 427-431 (2003)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Tanaka Y, et al.: "Protein Kinase C Promotes Apoptosis in LNCaP Prostate Cancer Cells through Activation of p38 MAPK and Inhibition of the Akt Survival Pathway"J Biol Chem. 278. 33753-33762 (2003)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Garcia-Bermejo ML, Leskow FC, Fujii T, Wang Q, Blumberg P, Ohba M, Kuroki T, Lee J, Marquez V, Kazanietz MG.: "DAG-lactones, a new class of PKC agonists, induce apoptosis in LNCaP prostate cancer cells by selective activation of PKCα"J Biol Chem. 277. 645-655 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Fujii_T, Sudo T, Sueyoshi S, Tanaka T, Fujita H, Shirouzu K, Ban S, Toyonaga A, Kato S, Yamana H.: "Clinicopathologic study of noevascularization and VEGF expression in superficial esophageal carcinoma."Int J Oncol. 21. 1181-1187 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Nakagawa S, Fujii_T, Yokoyama G, Kazanietz MG, Yamana H, Shirouzu K.: "Cell Growth Inhibition by All-trans Retinoic Acid in SKBR-3 Breast Cancer Cells : Involvement of protein kinase C α and extracellular signal-regulated kinase mitogen-activated protein kinase."Mol Carcinogen. 38. 106-116 (2003)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Fujii T, Nakagawa S, Hanzawa M, Sueyoshi S, Fujita H, Shirouzu K, Yamana H.: "Immunohistological Study of Cell Cycle-Related Factors, Oncogene Expression, Cell proliferation in adenocarcinoma of Barrett's Esophagus."Oncol Rep. 10. 427-431 (2003)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Tanaka Y, M.Gavrielides V, Mitsuuchi Y, Fujii T, Kazanietz MG: "Protein kinase C promotes apoptosis in LNCaP prostate cancer cells through the activation of p38 MAPK and inhibition of the Akt survival pathway."J Biol Chem. 278. 33753-33762 (2003)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Azuma K, Shichijo 5, Maeda Y, Nonaka Y, Fujii T, Koike K, Itoh K.: "Mutated p53 gene encodes a non-mutated epitope recognized by HLA-B4601-restricted and tumor-reactive CTLs at tumor site."Cancer Res. 15. 854-858 (2003)
  • Description: 「研究成果報告書概要(欧文)」より