Study on the protection by nitric oxide generated from L-Arginine

2003 Fiscal Year Final Research Report Summary

• Project/Area Number: 13671394
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Kochi University
• Principal Investigator: NONAMI Yoshiki Kochi University, Faculty of Medicine, Assistant professor, 医学部附属病院, 講師 (20164717)
• Co-Investigator(Kenkyū-buntansha): SASAGURI Sirou Kochi University, Faculty of Medicine, Professor, 医学部, 教授 (60196186) ; HAMASATO Sinzi Kochi University, Faculty of Medicine, Assistant, 医学部, 助手 (60228533)
• Project Period (FY): 2001 – 2003
• Keywords: cardiomyocytes / endothels / L-Arginine / nitric oxide

Research Abstract

Protective effects of L-arginine were evaluated in a cardiomyocytes and endothels got from rats model of low-volume anoxia and re-oxygenation. Cell cultures were subjected to 90 min of low-volume anoxia and 30 min of re-oxygenation. L-Arginine (0-0.5 mM) was administered during the pre-anoxic period or the re-oxygenation phase. Nitric oxide (NO) production, NO synthase (NOS) activity, cGMP levels, and cellular injury were assessed. To evaluate the effects of the L-arginine on cell signaling, the effects of the NOS antagonist NG-nitro-L-arginine methyl ester (L-NAME), No donor S-nitroso-N-acetyl-penicillamine (SNAP), guanylate cyclase inhibitor methylene blue, cGMP analog 8-bromo-cGMP were examined. This data indicate that low-volume anoxia and re-oxygenation might increase NOS activity and facilitated the conversion of L-arginine to NO, which provided protection against cellular injury in a dose-dependent fashion. In addition, the cardioprotective effects of L-arginine were achived by the activation of guanylate cyclase, leading to increased cGMP levels in rat heart cells. This action involves a glibenclamide-sensitive, NO-cGMP-dependent pathway.

Research Products

• [Journal Article] Nitric oxide generated from L-Arginine protects cardiomyocytes and endorhels from reperfusion injury produced by hypoxia and (2005)
  • Author(s): Nonami Y, Shiono N, Sasaguri S
  • Journal Title: J Heart Failure 12(in press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Study of the diagnostic difference between the clinical diagnostic criteria and results of immunohistochemical staining Of multiple primary lung cancers (2003)
  • Author(s): Nonami Y, Ohtuki Y, Sasaguri S
  • Journal Title: J Cardiovasc Surg 44 Pages: 661-5
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Study of the diagnostic difference between the clinical diagnostic criteria and results of immunohistochemicalstaining of multiple primary lung cancers. (2003)
  • Author(s): Nonami Y, Ohtuki Y, Sasaguri S
  • Journal Title: J Cardiovasc Surg 44 Pages: 661-665
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Nitric oxide generated from L-Arginine protects cardiomyocytes and endothels from reperfusion injury produced by hypoxia and re-oxygenation.
  • Author(s): Nonami Y, Shiono N, Sasaguri S
  • Journal Title: J Cardiac Failure (in press)
  • Description: 「研究成果報告書概要(欧文)」より