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2002 Fiscal Year Final Research Report Summary

Real-time visualization of partial liquid ventilation

Research Project

Project/Area Number 13671402
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Thoracic surgery
Research InstitutionJichi Medical School

Principal Investigator

ENDO Shunsuke  Jichi Medical School, Dep.of Surgery, Lecturer, 医学部, 講師 (10245037)

Co-Investigator(Kenkyū-buntansha) SAITO Noriko  Jichi Medical School, Dep.of Surgery, Lecturer, 医学部, 助手 (00347999)
OTANI Shinichi  Jichi Medical School, Dep.of Surgery, Lecturer, 医学部, 助手 (80337310)
SOHARA Yasunori  Jichi Medical School, Dep.of Surgery, Professor, 医学部, 教授 (60114097)
Project Period (FY) 2001 – 2002
KeywordsLiquid ventilation / ARDS / perfluorocarbon / ventilation-associated lung injury / fluorescence micrographs / pulmonary microcirculation / gas exchange
Research Abstract

Acute lung inury rat model were induced by tracheal instillation of not endotoxin but hydrochloric acid in this study, because endotoxin could not always induce lung injury in each rat model. To clarify an efficacy of partial liquid ventilation in the treatment of acute lung injury, we visualized and morphologically analyzed real-time alveolar recruitment in a rat lung injury model.
Male Wistar rats were divided into three groups : a group that underwent hydrochloric acid aspiration and mechanical gas ventilation (ALI, n=10), a group that underwent hydrochloric acid aspiration and partial liquid ventilation with perfluorocarbon beginning 90 mins after acid aspiration (PLV, n=10), and a group that did not undergo acid aspiration but that did undergo mechanical gas ventilation (Control, n=5). The average number of alveoli and the diameter of the largest functional alveoli in 10 high-power fields (1080 x 790 um) observed on fluorescence micrographs labeled albumin as a tracer, were measure … More d every 30 mins from 90 to 210 mins after the acid aspiration. Results showed that alveolar recruitment was marked immediately after partial liquid ventilation. Functional alveoli per high-power field decreased significantly in the ALI group (31.2±8.9), in comparison to that in the Control group (144±11.9). The number in the PLV group increased to 102.1±17.6 at 30 mins after partial liquid ventilation and to 130.3±19.1 until the end of the experiment. The diameter of the largest functional alveolus decreased from 103.7±16.3 um under mechanical gas ventilation before liquid ventilation to 86.5±7.4 um at 30mins after liquid ventilation. This value decreased further and remained constant until the end of the experiment at 76.3±6.5 um, which was equivalent to that in the Control group (75.1±9.4 um). Wet to dry lung weight ratios in the PLV group were smaller than those in the ALI group. The excellent alveolar recruitment suggests that liquid ventilation not only improves gas exchange but also ameliorates ventilator-associated lung injury.
These results have been reported at the annual meetings of Japan Surgical Society (2002), Japanese Association for Thoracic Society (2001) and were published in Microcirculation Annual (2002) and Surgery (2003). Less

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Endo S, et al.: "Surgical Outcome of Pulmonary Resection in Chronic Necrotizing Pulmonary Aspergillosis (CNPA)"Ann Thorac Surg. 72. 889-894 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Endo S, et al.: "Surgical Considerations for Pulmonary Actinomycosis"Ann Thorac Surg. 74. 185-190 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Endo S, et al.: "Scopulariopsis Fungus Ball"Ann Thorac Surg. 74. 926-927 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Endo S, et al.: "Real-time visualization of partial liquid ventilation via fluorescence microscopy"Microcirculation annual. 18. 123-124 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Endo S, et al.: "Real-time visualization of partial liquid ventilation in a model of acute lung injury"Surgery. 133. 207-215 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Endo S, et al.: "Management of Massive Hemoptysis in a Thoracic Surgical Unit"Eur J Cardio-Thorac Surg. 23. 467-472 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 遠藤俊輔, 他: "縦隔神経性腫瘍"医歯薬出版. (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Endo S, et al.: "Surgical Outcome of Pulmonary Resection in Chronic Necrotizing Pulmonary Aspergillosis (CNPA)"Ann Thorac Surg. 72. 889-894 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Endo S, et al.: "Surgical Considerations for Pulmonary Actinomycosis"Ann Thorac Surg. 74. 185-190 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Endo S, et al.: "Scopulariopsis Fungus Ball"Ann Thorac Surg. 74. 926-927 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Endo S, et al.: "Real-time visualization of partial liquid ventilation via fluorescence microscopy"Microcirculation annual. 18. 123-124 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Endo S, et al.: "Real-time visualization of partial liquid ventilation in a model of acute lung injury"Surgery. 133. 207-215 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Endo S, et al.: "Management of Massive Hemoptysis in a Thoracic. Surgical Unit"Eur J Cardio-Thorac Surg. 23. 467-472 (2003)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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