2002 Fiscal Year Final Research Report Summary
Angiogenic effect of eNOS gene-contained bone marrow cell transplantation
| Project/Area Number |
13671413
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Osaka Medical College |
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Principal Investigator |
HORIMOTO Hitoshi Osaka Medical College Faculty of Medicine Research Associate, 医学部, 助手 (70247849)
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| Co-Investigator(Kenkyū-buntansha) |
OKAWA Hirohisa Osaka Medical College Faculty of Medicine Research Associate, 医学部, 専攻医
HASEGAWA Shigeto Osaka Medical College Faculty of Medicine Assistant Professor, 医学部, 講師 (00247850)
SASAKI Shinjiro Osaka Medical College Faculty of Medicine Professor, 医学部, 教授 (10084881)
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| Project Period (FY) |
2001 – 2002
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| Keywords | angiogenesis / bone marrow cell / nitric oxide / ischemic tolerance |
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| Research Abstract |
Angiogenic effect of intra-arterial bone marrow cell transplantation into rat hindlimb ischemia
1) Rat hindlimb ischemia model was produced by removing unilateral femoral artery.
2) Bone marrow mononuclear cells of another rat were implanted into muscles in ischemic area.
3) Bone marrow mononuclear cells of another rat were implanted into artery close to ischemic area.
4) Intra-arterial bone marrow transplantation was shown to induce angiogenic effects similar to intra-muscular injection evident by angiography, thermogram and immunohistochemical analysis.
Angiogenic effect of intra-coronary bone marrow cell transplantation into rat ischemic myocardium
1) Rat ischemic myocardium was produced by ligating the left anterior descending coronary artery (LAD).
2) Two weeks after the LAD ligation, heart was excited and bone marrow mononuclear cells were implanted into coronary artery via aortic root. Then, the heart was transplanted into another rat abdomen by heterotopic heart transplantation method
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3) Two weeks after the heterotopic heart transplantation, heart was explanted and was subjected to Langendorff perfusion system to determine ischemic response against ischemia reperfusion insult and angiogenic effects.
4) There were no significant differences between bone marrow transplantation group and control group in terms of ischemic response, whereas significant angiogenic effects were observed in bone marrow transplantation group.
Effects of eNOS gene delivery on angiogenesis and ischemic response
1) Rat ischemic myocardium was produced by ligating the left anterior descending coronary artery (LAD).
2) Two weeks after the LAD ligation, heart was excised and bone marrow mononuclear cells and eNOS cDNA were implanted into coronary artery via aortic root. Then, the heart was transplanted into another rat abdomen by heterotopic heart transplantation method.
3) Two weeks after the heterotopic heart transplantation, hear was explanted and was subjected to Langendorff perfusion system to determine ischemic response against ischemia reperfusion insult and angiogenic effects.
4) The eNOS gene delivery significantly increased coronary flow compared to bone marrow cell transplantation alone and expression of NOS was determined in neovascular formation induced by bone marrow cell transplantation. Less
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