2002 Fiscal Year Final Research Report Summary
IMAGING OF MYOCARDIAL METABOLISM AND CORONARY MICROCIRCULATION DURING MYOCARDIAL ISCHEMIA/REPERFUSION
| Project/Area Number |
13671415
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Iwate Medical University (2002)
Kawasaki Medical School (2001) |
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Principal Investigator |
FUKUHIRO Yoshiaki Iwate Medical University Critical Care Medicine Assistant, 医学部, 助手 (20228927)
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| Co-Investigator(Kenkyū-buntansha) |
MOCHIZUKI Seiichi Kawasaki Medical School Medical Engineering Associate Prof., 臨床工学科, 助教授 (60259596)
OGASAWARA Yasuo Kawasaki Medical School Medical Engineering & System Cardiology Associate Prof., 医学部, 助教授 (10152365)
TNEMOTO Kazuo Kawasaki Medical School Thoracic & Cardiovascular Surgery Professor, 医学部, 教授 (90330547)
ENDO Shigeatsu Iwate Medical University Critical Care Medicine Professor, 医学部, 教授 (30160394)
KAWAZOE Kohei Iwate Medical University 3^<rd> Dept of Surgery Professor, 医学部, 教授 (50075561)
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| Project Period (FY) |
2001 – 2002
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| Keywords | Myocardial Ischemia / NADH / Coronary Microcirculation / Nitric Oxide / Hypoperfusion / Aging / Hypertension |
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| Research Abstract |
[Objective] To evaluate the contribution of nitric wide (NO) to myocardial energy metabolism during ischemia aid the effects of aging and hypertension on those parameters, we observed changes in myocardial surface NADH fluorescence. [Methods] Isolated rat hearts (WKY of 9, 16 wks ; SHR of 9, 16 wks) were Langendorff-perfused and were subjected to hypoperfusion followed by reperfusion. NADH images were real-timely video-recorded and time-course changes in NOx concentration in the coronary effluent was measured. {Results] In all isolated hearts, NADH fluorescence during hypoperfusion increased heterogeneously and then reached a steady level. This heterogeneous fluorescent pattern returned to the control level rapidly during reperfusion. Maximum changes of NADH fluorescent intensity during hypoperfusion were significantly greater in 16-wk SHR than that in other groups. Maximum NOx production during hypoperfusion significantly increased approximately in 9-wk WKY, while did not increase in other group. [Conclusions] Myocardial energy status changes heterogeneously during hypoperfusion/reperfusion. NO seems to be involved in regulation of the distribution of coronary microcirculation during hypoperfusion in the aspect of maintenance of the myocardial energy metabolism during hypoperfusion/reperfusion. The heterogenous distribution and time-course changes of NADH fluorescence were correlated with aging and hypertension and may reflect functional and structural changes in the coronary microcirculation and myocardial oxgen balance.
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