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2002 Fiscal Year Final Research Report Summary

Identification of glioma specific genes for the molecular marker

Research Project

Project/Area Number 13671465
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionKeio University

Principal Investigator

TODA Masahiro  Keio University, School of Medicine, Instructor, 医学部, 助手 (20217508)

Co-Investigator(Kenkyū-buntansha) KAWASE Takeshi  Keio University, School of Medicine, Professor, 医学部, 教授 (40095592)
KAWAKAMI Yutaka  Keio University, School of Medicine, Professor, 医学部, 教授 (50161287)
Project Period (FY) 2001 – 2002
Keywordsglioma / gene chip / SAGE / cancer / diagnosis / RT-PCR / EST
Research Abstract

Malignant gliomas are proliferative and invasive with poor prognosis. Although the recent progress of therapeutics has been shown in radiotherapy, chemotherapy, and gene therapy, the prognosis of glioma has not be improved. In order to identify specific molecules that can be targets for therapy and diagnostic markers of glioma, we used Affymetrix DNA chip technology and NCBI (National Center for Biotechnology Information) database including EST (expressed sequence tags) and SAGE (serial analysis of gene expression). First, we analyzed the gene expression in 11 glioma tissues compared with the average of that in 3 normal brain tissues using DNA chip containing 11000 Unigene clones. Sixteen genes were found to be expressed more than 3 times in all glioma tissues analyzed than normal brain. We then analyzed the expression of these selected genes by EST and SAGE programs in NCBI database. Among the 16 genes, five genes including the known glioma antigens, WAF1 and Tenascin C, showed to be overexpressed in cancer. By RT-PCR analysis of these 5 selected genes, we found a gene which shows a significantly higher expression in gliomas compared with normal brains. Using quantitative RT-PCR system with specific primers for this gene, we plan to establish a quick diagnosis method of glioma in the clinic

  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Toda M, Martuza RL, Rabkin SD: "Combination Suicide / cytokine gene therapy as adjuvants to a defective herpes simplex virus-based cancer vaccine"Gene Ther. 8. 332-339 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toda M, Iizuka Y, Yu W, Imai T, Ikeda E, Yoshida K, Kawase T, Kawakami Y, Okano H, Uyemura K: "Expression of the neural RNA-binding protein Musashi1 in human gliomas"Glia. 34. 1-7 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishii K, Toda M, Nakai Y, Asou H, Watanabe M, Nakamura M, Yato Y, Fujimura Y, Kawakami Y, Toyama Y, Uyemura K: "Increase of oligodendrocyte progenitor cells after spinal cord injury"J Neurosci Res. 65. 500-507 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Endo T, Toda M, Watanabe M, Iizuka Y, Kubota T, Kitajima M, Kawakami Y: "In situ cancer vaccination with a replication-conditional HSV for the treatment of liver metastasis of colon cancer"Gene Ther. 9. 142-148 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toda M, Iizuka Y, Kawase T, Uyemura K, Kawakami Y: "Immuno-viral therapy of brain tumors by combination of viral therapy with cancer vaccination using a replication-conditional"Cancer Gene Ther. 9. 356-364 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mikimi Y, Toda M, Watanabe M, Nakamura M, Toyama Y, Kawakami Y: "A simple and reliable behavioral analysis of locomotor function after spinal cord injury in mice"J Neurosurg. 97. 142-147 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toda M: "Immuno-viral therapy as new approach for the treatment of brain tumor"Drug News Perspect. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toda M, Kawakami Y: "Immunotherapy of brain tumors"Curr Pharm Des. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 戸田正博, 飯塚幸彦, 植田良, 吉田一成, 河瀬 斌, 河上 裕: "グリオーマ抗原分子を用いた新しい診断法の開発"Neuro-Oncology. 11. 93-94 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 戸田正博: "悪性神経膠腫に対する樹状細胞を用いたワクチン療法"Medical Briefs in Brain & Nerve. 12. 93-94 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 戸田正博: "腫瘍ワクチン療法"Clinical Neuroscience. 21. 543-543 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 戸田正博: "Anuual Review免疫"中外医学社. 290 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 戸田正博, 飯塚幸彦, 植田 良, 吉田一成, 河瀬 斌, 河上 裕: "ポストシークエンス時代における脳腫瘍の研究と治療"財団法人九州大学出版会. 561 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 戸田正博: "先端医療シリーズ18 脳神経外科 脳腫瘍の最新医療"先端医療技術研究所. 398 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toda,M, Martuza,R,L, Rabkin,S,D: "Combination Suicide / cytokine gene therapy as adjuvants to a Defective herpes simplex virus-based cancer vaccine"Gene Ther. 8. 332-339 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toda,M, Iizuka,Y, Yu,W, Imai,T, Ikeda,E, Yoshida,K, Kawase,T, Kawakami,Y, Okano,H, Uyemura,K: "Expression of the neural RNA-binding protein Musashil in human gliomas"Glia. 34. 1-7 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishii,K, Toda,M, Nakai,Y, Asou,H, Watanabe,M, Nakamura,M, Yato,Y, Fujimura,Y, Kawakami,Y, Toyama,Y, Uyemura,K: "Increase of oligodendrocyte progenitor cells after spinal cord injury"J Neurosci Res. 65. 500-507 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Endo,T, Toda,M, Watanabe,M, Iizuka,Y, Kubota,T, Kitajima,M, Kawakami,Y: "In situ cancer vaccination with a replication-conditional HSV for the treatment of liver metastasis of colon cancer"Cancer Gene Ther. 9. 142-148 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toda,M, Iizuka,Y, Kawase,T, Uyemura,K, Kawakami,Y: "Immuno-viral therapy of brain tumors by combination of viral therapy with cancer vaccination using a replication-conditional HSV"Cancer Gene Ther. 9. 356-364 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mikimi,Y, Toda,M, Watanabe,M, Nakamura,M, Toyama,Y, Kawakami,Y: "A simple and reliable behavioral analysis of locomotor function after spinal cord injury in mice"J Neurosurg. 97. 142-147 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toda,M: "Immune-viral therapy as new approach for the treatment of brain tumor"Drug News Perspect. in press.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toda,M, Kawakami,Y: "Immunotherapy of brain tumors"Curr Pharm Des. in press.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toda,M, Iizuka,Y, Ueda,R, Yoshida,K, Kawase,T, Kawakami,Y: "Development of new diagnostic methods for glioma using their antigens"Neuro-Oncology. 11. 93-94 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toda,M: "Cancer vaccine with dendritic cells for the treatment of glioma"Medical Briefs in Brain & Nerve. 12. 93-94 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toda,M: "Cancer vaccine"Clinical Neuroscience. 21. 543-543 (2003)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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