The experimental study for creating an artificial nerve conduit with Schwann cells

2003 Fiscal Year Final Research Report Summary

• Project/Area Number: 13671488
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: University of Tsukuba
• Principal Investigator: NISHIURA Yasumasa University of Tsukuba, Institute of Clinical Medicine, Assiatant Professor (80208131)
• Co-Investigator(Kenkyū-buntansha): OCHIAI Naoyuki University of Tsukuba, Institute of Clinical Medicine, Professor (30134563) ; CHIN Kokuhei Advanced Industrial Science and Technology, Tissueengineering research center, ティッシュエンジニアリング研究センター, Principal researcher ; SMI Yoshihiko TEIJIN Holding Company, Engineering research group, Group leader
• Project Period (FY): 2001 – 2003
• Keywords: nerve regeneration / artificial nerve conduit / Schwann cell / bone marrow cell / peripheral nerve defect

Research Abstract

Purpose : We performed experimental studies of bridging a nerve gap of a rat sciatic nerve by absorbable artificial tubes with Schwann cells or bone marrow cells and investigated the usefulness of them.
Methods : Experimet I : We used a macroporous collagen tube and bridged a 15mm long nerve defect (n=12). Experiment II : We used a PLA tube and bridged a 15mm long nerve defect. PLA group (n=6), laminin-coated PLA group (n=3), laminin-coated PLA with bone marrow monocyte cells group(n=3) were made. Experiment III : We used a PLA tube and bridged a 15mm long nerve defect. PLA packed with collagen gel group (n=3), PLA packed with collagen gel with cultured Schwann cells group (n=3), PLA packed with collagen gel with cultured bone marrow stromal cells group (n=3) were made.
Results : Experimet I : Eight weeks postoperatively the continuity of a tube was held in all cases macroscopically, but microscopically nerve regeneration was seen at the center of the tube in three cases. Disappearance of porous architecture seemed to prevent axons from regenerating. Experiment II : Eight weeks postoperatively nerve regeneration was not seen at the center in PLA group and laminin-coated PLA group. Nerve regeneration was seen at the center in two cases and at the distal part in a case of of bone marrow monocyte cells group. Experiment III : Eight weeks postoperatively nerve regeneration was not seen at the center in PLA group and cultured bone marrow stromal cell group. Nerve regeneration was seen at the center in a case of cultured Schwann cells group.
Conclusion : The PLA tube that we invented was considered to be useful as a nerve conduit because of keeping tubal architecture at some periods. As large amount of cultured Schwann cells needs the sacrifice of a larger peripheral nerve, the use of cultured Schwann cells is difficult. Alternatively, it was suggested that bone marrow monocyte cells facilitate nerve regeneration.

Research Products

• [Patent(Industrial Property Rights)] 神経再生材料 (2005)
  • Inventor(s): 北薗英一, 兼子博章, 鷲見芳彦, 西浦康正, 吉井雄一, 落合直之
  • Industrial Property Rights Holder: 帝人株式会社および筑波大学
  • Industrial Property Number: 特願2005-369355(公開番号2007-167366)
  • Filing Date: 2005-12-22
  • Description: 「研究成果報告書概要(和文)」より