Osteogenic capacity of vascularized periosteal graft combined with cultured mesenchymal stem cells

2003 Fiscal Year Final Research Report Summary

• Project/Area Number: 13671535
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Nara Medical University
• Principal Investigator: YAJIMA Hiroshi Nara Medical University, Orthopaedic surgery, Associate Professor, 医学部, 助教授 (20221640)
• Co-Investigator(Kenkyū-buntansha): KOBATA Yasunori Nara Medical University, Orthopaedic Surgery, Research Associate, 助手 (30347554) ; YOSHIKAWA Takaaki Nara Medical University, Pathology, Assistant Professor, 講師 (90275347)
• Project Period (FY): 2001 – 2003
• Keywords: periosteum / vascualrized / bone graft / mesenchymal stem cell / sltured cell / bone marrow / hybrid / collagen

Research Abstract

The purpose of our study was to analyze the osteogenic capacity of the vascularized * using an experimental model of the vascularized tibial periosteum and various cerai * in rats. In addition, we investigate the efficacy of cultured mesenchymal stem cells ******* vascularized periosteal graft model as well.
Hydroxyapatite or β TCP as a scaffold was wrapped with the vascularized perio * composite was grafted under skin of thigh area. Marked bone formation was observed in both hydroxyapatite and β TCP group at 2 weeks or later. However, in the vascularized fascia-ceramics composite group (control group), no bone formation was observed histologically. Biochemical analysis using alkaline phosphatase and osteocalsin also indicate the same results. On the other hand, from in vitrostudy of osteoinduction by collagen apatite composite combined with cultured MSCs, osteoinduction potential of the collagen apatite composite with MSCs group was significantly higher than apatite only group as a control. Based on the results of our previous series, we performed in vivo study using vascularized periosteal graft model combined with cultured MSCs in mature rats. A collagen apatite composite was selected as the scaffold. In MSCs impregnated group, much more bone formation was observed than non-MSCs group. In conclusion, even in the matured rat which has little potential of osteogenesis, combained method of vascularized periosteum and cultured MSCs has high osteogenic capacity. And so we consider this combined method must be useful in the treatment of refractory non-union of the bones in human.