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2003 Fiscal Year Final Research Report Summary

Identification of a binding site of general anesthetics on the tandem pore domain potassium channels

Research Project

Project/Area Number 13671567
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionNIIGATA UNIVERSITY

Principal Investigator

YAMAKURA Tomohiro  NIIGATA UNIVERSITY, Graduate School of Medical and Dental Sciences, Associate Professor, 大学院・医歯学総合研究科, 助教授 (80272847)

Project Period (FY) 2001 – 2003
KeywordsGeneral anesthetics / Potassium channels / Voltage-dependent / Inwardly rectifying / G protein-coupled / Molecular biology
Research Abstract

The ERG1,ELK1,and KCNQ2/3 voltage-gated potassium channels were resistant to 2 minimum alveolar concentrations(MAC) of all general anesthetics tested(halothane, isoflurane, enflurane, F3,F6,pentobarbital, propofol, etomidate, alphaxalone, ketamine, ethanol, and hexanol). On the other hand, among inwardly rectifying potassium channels, GIRK channels were inhibited by volatile anesthetics, but not by intravenous anesthetics. The neuronal-type GIRK1/2 channels were inhibited by 2 MAC of halothane, isoflurane, enflurane, and F3, whereas the cardiac-type GIRK1/4 channels were inhibited only by F3. Conversely, IRK1 and ROMK1 channels were completely resistant to all anesthetics tested. Current responses of GIRK2 channels activated by m-opioid receptors were also inhibited by halothane. Thus, among inwardly rectifying potassium channels, GIRK channels, especially those composed of the GIRK2 subunit(neuronal subunit), are suggested to be sensitive to volatile anesthetics. Nitrous oxide (〜0.6 atmosphere) slightly but selectively potentiated GIRK channels(GIRK1/2,GIRK1/4, GIRK2 channels) by 〜10%. GIRK2 channels activated by mu-opioid receptors were also potentiated by nitrous oxide. Results of chimeric and multiple amino acid mutations suggest that the region containing the trans membrane domains, but not the pore-forming domain, may be involved in determining differences in anesthetic sensitivity between GIRK and IRK channels. Although inhibition of GIRK channel function by anesthetics is unlikely to account for the anesthetic actions, it may contribute to some excitatory side effects.

  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] Eger H EI, et al.: "Relevant concentrations of inhaled anesthetics for in vitro studies of anesthetic mechanisms"Anesthesiology. 94(5). 915-921 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山倉智宏: "Effects of general anesthetics on G-protein-coupled inwardly rectifying potassium channels"麻酔と蘇生Anesthesia and Resuscitation. 37(3・4). 89-90 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hara K, et al.: "Mutational analysis of ethanol interactions with G-protein-coupled inwardly rectifying potassium channels"Alcohol. 21(1). 5-8 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ikeno K, et al.: "The Lurcher mutation reveals Ca^<2+> permeability and PKC modification of the GluRd channels"Neurosci Res. 41(2). 193-200 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mascia MP, et al.: "Does acetaldehyde mediate ethanol action in the central nervous system?"Alcohol Clin Exp Res. 25(11). 1570-1575 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Qi S, et al.: "Sublethal cerebral ischemia inhibits caspase-3 activation induced by subsequent prolonged ischemia in the C57Black/Crj6 strain mouse"Neurosci Lett. 315(3). 133-136 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamakura T, et al.: "Anesthetics and ion channels : molecular models and sites of action"Annu Rev Pharmacol Toxicol. 41. 23-51 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamakura T, et al.: "Differential effects of general anesthetics on G-protein-coupled inwardly rectifying and other potassium channels"Anesthesiology. 95(1). 144-153 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Zhang Y, et al.: "Luciferase as a model for the site of inhaled anesthetic action"Anesth Analg. 93(5). 1246-1252 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimizu M, et al.: "Propofol enhances GABA_A receptor-mediated presynaptic inhibition in human spinal cord"Neuroreport. 13(3). 357-360 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Zhan RZ, et al.: "Ischemic preconditioning is capable of inducing mitochondrial tolerance in the rat brain"Anesthesiology. 97(4). 896-901 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Petrenko AB, et al.: "The role of N-methyl-D-aspartate (NMDA) receptors in pain : A review"Anesth Analg. 97(4). 1108-1116 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Petrenko AB, et al.: "Unaltered pain-related behavior in mice lacking NMDA receptor GluRε1 subunit"Neurosci Res. 46(2). 199-204 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tobita T, et al.: "Diagnosis of spinal disease with ultrafine flexible fiberscopes in patients with chronic pain"Spine. 28(17). 2006-2012 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tobita T, et al.: "The effects of isoflurane on conditioned inhibition by dorsal column stimulation"Anesth Analg. 97(2). 436-441 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamakura T: "Editorial : Volatile anesthetic antagonism by long-chain free fatty acids"J Anesth. (in press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山倉智宏: "麻酔薬の作用機序-イオンチャネル分子への作用と麻酔作用の連関性-"新潟医学会雑誌. (in press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山倉智宏: "全身麻酔薬とグルタミン酸受容体-分子作用と麻酔作用-"Pharmacoanesthesiology. (in press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Petrenko AB, et al.: "Recent Advances in Anaesthesia and Intensive Care"Greenwich Medical Media Limited. 177-207 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Eger II El, et al.: "Relevant concentrations of inhaled anesthetics for in vitro studies of anesthetic mechanisms"Anesthesiology. 94(5). 915-921 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hara K, et al.: "Mutational analysis of ethanol interactions with G-protein-coupled inwardly rectifying potassium channels"Alcohol. 24(1). 5-8 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ikeno K, et al.: "The Lurcher mutation reveals Ca^<2+> permeability and PKC modification of the GluRd channels"Neurosci Res. 41(2). 193-200 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mascia MP, et al.: "Does acetaldehyde mediate ethanol action in the central nervous system?"Alcohol Clin Exp Res. 25(11). 1570-1575 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Qi S, et al.: "Sublethal cerebral ischemia inhibits caspase-3 activation induced by subsequent prolonged ischemia in the C57Black/Crj6 strain mouse"Neurosci Lett. 315(3). 133-136 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamakura T, et al.: "Anesthetics and ion channels : molecular models and sites of action"Annu Rev Pharmacol Toxicol. 41. 23-51 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamakura T, et al.: "Differential effects of general anesthetics on G-protein-coupled inwardly rectifying and of her potassium channels"Anesthesiology. 95(1). 144-153 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Zhang Y, et al.: "Luciferase as a model for the site of inhaled anesthetic action"Anesth Analg. 93(5). 1246-1252 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimizu M, et al.: "Propofol enhances GABA_A receptor-mediated presynaptic inhibition in human spinal cord"Neuroreport. 13(3). 357-360 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Zhan RZ, et al.: "Ischemic preconditioning is capable of inducing mitochondria! tolerance in the rat brain"Anesthesiology. 97(4). 896-901 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Petrenko AB, et al.: "The role of N-methyl-D-aspartaie(NMDA) receptors in pain : A review"Anesth Analg. 97(4). 1108-1116 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Petrenko AB, et al.: "Unaltered pain-related behavior in mice lacking NMDA receptor GluRε1 subunit"Neurosci Res. 46(2). 199-204 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tobita T, et al.: "Diagnosis of spinal disease with ultra fine flexible fiberscopes in patients with chronic pain"Spine. 28(17). 2006-2012 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tobita T, et al.: "The effects of isoflurane on conditioned inhibition by dorsal column stimulation"Anesth Analg. 97(2). 436-441 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamakura T: "Editorial : Volatile anesthetic antagonism by long-chain free fatty acids"J Anesth. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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