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2003 Fiscal Year Final Research Report Summary

Expression of the Water Channel in the Central Nervous System and Mechanisms of the Regulation of the Expression

Research Project

Project/Area Number 13671603
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionNagoya City University

Principal Investigator

TAKEUCHI Akinori  Nagoya City University, Graduate School of Medical Sciences, Associate Professor, 大学院・医学研究科, 助教授 (50254305)

Co-Investigator(Kenkyū-buntansha) ASAI Kiyofumi  Nagoya City University, Graduate School of Medical Sciences, Professor, 大学院・医学研究科, 教授 (70212462)
SOBUE Kazuya  Nagoya City University, Graduate School of Medical Sciences, Research Associate, 大学院・医学研究科, 助手 (90264738)
Project Period (FY) 2001 – 2003
KeywordsEdema / Aquaporin / Astrocytes / Brain / Culture / Trauma
Research Abstract

Aquaporins, water channels, are a family of water-selective transporting proteins, that play an important role in water transport in the brain. Firstly, the expression of AQP family was investigated thoroughly in the central nervous system. In the brain, we detected the transcripts of AQP3, AQP4, AQP5, AQP8, AQP9 in astrocytes using the reverse transcription-PCR. These results suggest that AQP in astrocytes have roles in not only water transport but also other unknown functions. Secondly, regulatory mechanisms of AQP expressions were also investigated. The expression of AQP4 was down-regulated by protein kinase C (PKC). The expression of AQP5 was down-regulated by protein kinase A (PKA). The expression of AQP9 was up-regulated by PKA and down-regulated PKC. The p38 mitogen-activated proteinkinase (p38 MAPK) regulated the expression of AQP4 and AQP9. These results suggest that the expressions of AQPs are intricately regulated according to the conditions. Third, we examined the changes of AQP expressions under hypoxic stress. The expressions of AQP4 and AQP9 were up-regulated by hypoxia, but precise mechanisms for their regulation are still unclear. Further studies are needed to clarify the mechanisms.
New therapeutic method for brain edema may be developed from our basic data. The agent of regulation of AQP expression or AQP antibodies might be new medicines for brain edema.

  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Yamamoto N: "Alterations in the expression of the AQP family in cultured rat astrocytes during hypoxia and reoxygenation"Brain Res.Mol.Brain Res.. 90. 26-38 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamamoto N: "Differential regulation of aquaporin expression in astrocytes by protein kinase C"Brain Res.Mol.Brain Res.. 95. 110-116 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamamoto N: "Differential regulation of aquaporin-5 and -9 expression in astrocytes by protein kinase A"Brain Res.Mol.Brain Res.. 104. 96-102 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Arima H: "Hyperosmotic mannitol stimulates expression of aquaporin 4 and 9 through a p38 mitogen activated kinase-dependent pathway in rat astrocytes."J.Biol.Chem.. 278. 44525-44534 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujita Y: "Effect of Mild Hypothermia on the Expression of Aquaporin Family in Cultured Rat Astrocytes under Hypoxic Condition."Neurosci.Res.. 47. 437-444 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamamoto N et al.: "Alterations in the expression of the AQP family in cultured rat astrocytes during hypoxia and reoxygenation."Brain Res Mol Brain Res. 90. 26-38 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yaneda K et al.: "Regulation of aquaporin-4 expression in astrocytes."Brain Res Mol Brain Res. 89. 94-102 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamamoto N et al.: "Differential regulation of aquaporin expression in astrocyte by protein kinase C."Brain Res Mol Brain Res. 95. 110-16 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamamoto N et al.: "Differential regulation of aquaporin-5 and -9 expressession in astrocytes by protein kinase A"Brain Res Mol Brain Res. 104. 96-102 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Arima H et al.: "Hyperosmotic mannitol stimulates expression of aquaporin 4 and 9 through a p38 mitogen activated kinase-dependent pathway in rat astrocytes."J Biol Chem. 278. 44525-34 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujita Y et al.: "Effect of Mild Hypothermia on the Expression of Aquaporin Family in Cultured Rat Astrocytes under Hypoxic Condition."Neurosci Res. 47. 437-44 (2003)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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