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2003 Fiscal Year Final Research Report Summary

Analysis of opioid receptors and substance P activity in signal transduction and inflammation

Research Project

Project/Area Number 13671615
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionJUNTENDO UNIVERSITY

Principal Investigator

NISHIMURA Kinya  Juntendo University School of Medicine, associate Professor, 医学部, 助教授 (80164581)

Co-Investigator(Kenkyū-buntansha) WARABI Kengo  Juntendo University School of Medicine, assistant Professor, 医学部, 助手 (30311989)
Project Period (FY) 2001 – 2003
KeywordsG protein / Spinorphin / LTP / Stnatum / substance P
Research Abstract

We found an endogenous factor that modulated enkephalin-degrating activity and purified it from bovine spinal cord based on its inhibitory activity toward enkephalin-degrading enzymes. Structural analysis revealed the factor to be Leu-Val-Tyr-Pro-Trp-Thr and it was named spinorphin. It has been found that spinorphin inhibited the activity toward various enkephalin-degrating enzymes from monkey brain, especially dipeptidyl peptidase III. Recently we found that this inhibitory significantly inhibited bradykin-induced nociceptive flexor responses. We also have reported roles for spinorphin in inflammation. Spinorphin significantly inhibited the functions of polymorphonuclear neutrophils by suppressing the binding of fMLF to its receptor on PMNs. The possible role of spinorphin as reglulators in pain and inflammation will be discussed.
G protein coupled receptor kinases (GRKs) phosphorylate agonist-occupied G protein-coupled receptors, leading to receptor desensitization. We investigated whether human substance P receptor is a substrate of GRK5. We report that human Substance P receptor is phosphorylated by purified GRK5. The phosphorylation has a high stoichiometry and a low Km. These data provide the first evidence that human substance P receptor is a substrate of GRK5.
Although the importance of Ach in the learning and memory processes has been point out in the stratum as well as the cerebral cortex. The aim of our study was to answer this question using the striatal cholinergic inter-neurons and to elucidate the mechanism of synaptic plasticity in them. The results obtained here indicate the following. Electrical stimulation of cortico/thalamostriatal pathway frequently evokes a depolarizing and hyperpolarizing postsynaptic potential in the cholinergic inter-neurons that is composed of a cortic/thalamostriatal glutamatergic EPSP and an intrastriatally evoked disynaptic GABAergic IPSP, respectively.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Yamamoto Y, Nishimura K. et al.: "Spinorhin as an endogenous inhibitor of Enkephalin-degrading enzyme : Roles in Pain and inflammation."Current protein & peptide science. 3. 587-599 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzuki T, Nishimura K. et al.: "Dopamine-dependent synaptic plasticity in the striatal cholinergic interneurons."J Neouroscience. 21. 6492-6501 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Warabi K, Nishimura K. et al.: "Human substance P receptor undergoes agonist-dependent phosphorylation by G-protein-coupled receptor kinase 5"FEBS lett.. 521. 140-144 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 西村欣也, 釘宮豊城: "心不全とエビデンス"臨床麻酔. 27. 1451-1457 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 西村欣也, 蕨 謙吾, 他: "オピオイド受容体とGタンパク質-その薬理と臨床-"臨床麻酔. 27. 1785-1796 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山口敬介, 西村欣也, 他: "細胞内情報伝達-レセプターから核まで-"臨床麻酔. 26. 1233-1243 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamamoto Y, Nishimura K. et al.: "Spinorhin as an endogenous inhibitor of Enkephalin-degrading enzyme: Roles in Pain and inflammation."Current protein and peptide science. 3. 587-599 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki T, Nishimura K. et al.: "Dopamine-dependent synaptic plasticity in the Striatal cholinergic interneurons."J Neuroscience. 21. 6492-6501 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Warabi k, Nishimura K. et al.: "Human substance p receptor undergoes agonist-dependent Phosphorylation by G protein-coupled receptor kinase 6 in Vitro"FEBS. 521. 140-144 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nishimura K, Kugimiya T.: "Heart failure: Based on Evidence."J.Clin.Anesth.Jpn. 27. 1451-1457 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nishimura K, Warabi K. et al.: "Review of intravenous anesthetics and opioids Opioid receptor mediated signaling and G protein"J.Clin.Anesth.Jpn. 27. 1785-1796 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamaguchi K, Nishimura K. et al.: "Pathways connecting G protein-coupled receptors To the nucleus through mitogen-activated protein kinase cascades."J.Clin.Anesht.Jpn. 26. 1233-1243 (2002)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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