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2002 Fiscal Year Final Research Report Summary

Role of co-stimulatory signal in long-term acceptance of human kidney allogrfts.

Research Project

Project/Area Number 13671646
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionNagoya University

Principal Investigator

ONO Yoshinari  Nagoya University, Graduate School of Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (00301218)

Co-Investigator(Kenkyū-buntansha) YOSHINO Yasushi  Nagoya University, University Hospital, Research Associate, 医学部附属病院, 助手 (70324415)
HATTORI Ryouhei  Nagoya University, University Hospital, Assistant Professor, 医学部附属病院, 講師 (20324410)
Project Period (FY) 2001 – 2002
Keywordsco-stimulatory signal / human kidney transplantation / immunological tolerance / chronic rejection
Research Abstract

To evaluate the role of co-stimulatory signal in long-term human kidney allograft acceptance, CD 2, CD 11a and CD 28 expression on peripheral lymphocytes of human kidney transplant patients were examined in 67 kidney transplant patients, 14 healthy volunteers and 10 hemodialysis patients. Only CD 28 was remarkably depressed in the kidney transplant patients, which was significantly correlated with the duration of graft survival. In age matched healthy volunteers and hemodialysis patients did not have depressed expression of co-stimulatory antigens. Depressed CD28 was induced mainly on CD4 positive T cells. The CD28-negative CD 4-positive T cell did not express CD 25, CD69, V alpha 24 nor CTLA-4. RT-PCR and southern blotting analysis for repertoire of the V beta chain indicated remarkable deviation in the CD28-negative CD 4-positive T cell. Also, it had elevated expression of INF-gammer and TGF-beta 1, but not IL-4 and IL-10. In MLC study between kidney transplant patients, donors and third party, donor specific suppression was observed in patients with the more CD28-negative CD 4-positive T cells. In prospective 3 year-survey of the rate of CD28-negative CD 4-positive T cell/CD 4-positive T cell in 74 long-term kidney transplant patients, it was lower in the recipients with chronic rejection.
In conclusion, the CD28-negative CD 4-positive T cell might be induced by continuous stimulation of donor antigens and might play a significant role in suppression of rejection.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] 小野佳成: "移植腎に生ずる非免疫学的障害"Annual Review 腎臓 2000(中外医学社). 169-174 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 小野佳成: "慢性拒絶反応の克服"医学のあゆみ. 196・13. 991-994 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shinichi Ohshima: "Long-Term Outcome of Cadaver Kidney Transplants from Non-Heart-Beating"Transplantation Proceeding. 33. 3764-3768 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazuo Mizutani: "Use of Marginal Organs from Non-Heart-Beating Cadaveric Kidney Donors"Transplantation. 72. 1376-1380 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masashi Kato: "Characterization of CD28^-CD4^+ T Cell in Living Kidney Transplant Patients With Long-Term Allograft Acceptance"Human Immunology. 62. 1335-1345 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Ono: "Non-immunogic damage on kidney allograft"Annual Review Kidney. 2000 (中外医学社). 169-174 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Ono: "Chronic allograft rejection"Igaku no Ayumi. 991-994 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Ohshima, Y.Ono, R.Hattori, N.Fukuhara, T.Kinukawa, S.Yamada, O.Kamihira, N.Nishiyama: "Long-Team Outcome of Cadaver Kidney Transplants from Non-Heart-Beating Donors"Transplantation Proceeding. 33. 3764-3768 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Mizutani, Y.Ono, T.Kinukawa, R.Hattori, N.Nisjiyama,O.Kamihira, S.Ohshima: "Use of Marginal Organs from Non-Heart-Beating Cadaveric Kidney Donors"Transplantation. 72. 1376-1380 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Ksto, T.Kinukawa, Y.Ono, R.Hattori, S.Ohshima, Y.Yoshikai: "Characterization of CD28^- CD4^+ T Cell in Living Kidney Transplant Patients With Long-Term Allograft Acceptance"Human Immunology. 62. 1335-1345 (2001)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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