2002 Fiscal Year Final Research Report Summary
Search for new target antigens of cancer by the SEREX method using cancer patient's serum containing antibodies
| Project/Area Number |
13671680
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | AICHI MEDICAL UNIVERSITY |
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Principal Investigator |
YOSHIKAWA Kazuhiro DEPT. OF PATHOLOGY, LECTURER, 医学部, 講師 (60109759)
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| Co-Investigator(Kenkyū-buntansha) |
UEMURA Masatsugu Nara Medical Univ, DEPT. OF UROLOGY, LECTURER, 講師 (90213397)
SAGA Shinsuke DEPT. OF PATHOLOGY, PROFESSOR, 医学部, 教授 (40144141)
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| Project Period (FY) |
2001 – 2002
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| Keywords | Urological tumor / SEREX |
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| Research Abstract |
The SEREX method is a system to identify unique antibodies and antigens for cancer patients using autologouse cancer tissue and serum and many researchers had found many unique antigens. These results showed that many of these antigens detected expressed in Testis. So, these antigens were named Cancer testis (CT) antigen. These antigens are useful targets to induce cellular immunity to treat cancers, since, on the Spermatogenic cells surface, HLA-class I antigen is not expressed.
In this study, we collected cancer tissues and autologouse serums of a prostate and 5 renal cancer, and serums of 3 prostate and 10 renal cancers. We tried to make an expression libraries using mRNAs from prostate cancer tissues and we found that the titer of these libraries were 2.5x10^6 pfu.
And we made an expression library using one tissue of two testis obtained. We tested the usefulness of this testis library using cancer serum, which was confirmed to contained some antibodies reacted to cancer tissues, and could get many positive cloned. We determined nucleotide sequences of cDNA fragments in positive clones and found that we got 7 unique clones and many positive clones were same clones. Now, we can't open the name of unique genes which contained come antigens detected in auto-immune disease.
These results showed that the our original libraries were useful tools to detect antigens recognized with cancer patients serum. So, we continue to screen the library using several cancer serums.
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