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2002 Fiscal Year Final Research Report Summary

Human follicular development flollowing xenotransplantation of human pnarian cortex into immune deficient mice

Research Project

Project/Area Number 13671688
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionTohoku University

Principal Investigator

TERADA Yukihiro  Tohoku University, Hospital, Associate Professor, 医学部附属病院, 助教授 (10260431)

Co-Investigator(Kenkyū-buntansha) MURAKAMI Takashi  Tohoku University, Graduate School of Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (20240666)
Project Period (FY) 2001 – 2002
KeywordsOvarian transplatation / Human follicle / Immune dificient mouse / Steroidgenic enzymes
Research Abstract

We studied the immunohistochemical expression and localization of steroidogenic enzymes in the graft of non-pathological human ovary following xenogenic transplantation into a non-obese diabetic severe combined immune deficient (NOD-SCID) mice. Human follicles following xenotransplantation into NOD-SCID mice were studied by immunohistochemistry antibodies against steroidgenic enzymes. In the pre antral follicles of these grafts, steroidogenic enzymes, p450 scc,p450 c17 and 3β-HSD were present in only the the theca cell layer, observations which are consistent with the findings of non-pathological human ovarian cortex. Cytochrome p450 aromatase and estrogen receptor, however, were not detected in these grafts, and these follicles did not possess specific feature of a dominant follicle. These findings suggest that the expression of steroidogenic enzymes in humnan follicles following xenogenic transplantation into NOD-SCID mice. is similar to that of non-pathological human ovaries. Howeve … More r, these follicles do not possess features of dominant follicles. which are known to develop into the corpus luteum. Furthermore, we succeed the successful generation of mature human graafian follicles following xenotransplantation of a human ovarian cortex into a NOD/SCID/g cnull mouse. Ten weeks after transplantation, we stimulated the ovary tissue through daily intraperitoneal injections of human menopausal gonadotropin for 14 days. Ovarian grafts were removed from recipient mice twelve weeks after transplantation. In 2 out of 8 transplanted mice. human graafian follicles l6-18 mm in diameter were obtained from transplanted tissues within the ovarian bursa. High levels of cytochrome p450 aromatase and estroqen receptor expression were observed in both the granulosa and the human graafian follicle theca layer developed in tne murine ovarian bursa. These results suggest that these follicles have similar characteristics as the dominant follicles of a normal premenopausal cycling human ovary. Less

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Sato Y, Terada Y, et al..: "Immunohistochemical localization of steroidogenic enzymes in human follicle following xenotransplantation of the human ovarian cortex into NOD-SCID mice"Molecular Reproduction and Development. 65. 67-72 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Terada Y. et al.: "Intracytoplasmie sperm injection (ICSI) : Stilleto Conception or a Stab in the Dark. (Review)"Archieves of Audiology. 49. 169-177 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakamuta S, Terada Y.et al.: "Anakysis of the human sperm centrosomal function and the oocyte activation in a case of globozoospermia by ICSI into bovine oocytes"Human Reproduction. 17. 2930-2934 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Rawe V, Terada Y, et al.: "A pathology of the sperm centriole responsible for defective sperm aster formation, sygamy and cleavage"Human Reproduction. 17. 2344-2349 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Terada Y, et al.: "Human sperm aster formation after intracytoplasmic sperm injection with rabbit and bovine eggs"Fertil Sieril. 77. 1283-1284 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sato Y., Terada Y., Utsunomiya H., Koyanagi Y, Okamura K.: "Immunohistochemical localization of steroidogenic enzymes in human follicle following xenotransplantation of the human ovarian cortex into NOD-SCID mice."Molecular Reproduction and Development. 65. 67-72 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Terada Y., Nakamura S., Morita J., Hewitson L., Sinerly CR., Murakami T., Yaegashi N., Schatten G., Okamura K.: "lntracytoplasmic sperm injection (ICSI) : Stilleto Conception or a Stab in ths Dark."Archieves ofl Andrology (Review). 49. 169-177 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakamura S., Tearada Y., Horiuchi T., Emuta C., Murakami T., Yaegashi N., Okamura K.: "Anakysis of the human sperm centrosomal function and the oocyte activation in a case of globozoospermia by ICSI into bovine oocytes."Human Reproduction. 17. 2930-2934 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Rawe V., Terada T., Nakamura S., Chillik CF., Brugo Olmedo S., Chemes HE.: "A pathology of the sperm centriole responsible for defective sperm aster formation, sygarmy and cleavage."Human Reproduction. 17. 2344-2349 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Terada Y., Nakamura S., Hewitson L., Sinerly CR., Horiuchi T., Murakami T., Okamura K., Schatten G.: "Human sperm aster formation after intracytoplasmic sperm injection with rabbit and bovine eggs."Fertil Steril. 77. 1283-1284 (2002)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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