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2002 Fiscal Year Final Research Report Summary

Role of molecules expressed in human extravillous trophoblasts on the regulatory mechanism of cancer invasion

Research Project

Project/Area Number 13671710
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionKyoto University

Principal Investigator

HIGUCHI Toshihiro  KYOTO UNIVERSITY, Graduate school of Medicine, Assistant, 医学研究科, 助手 (00283614)

Co-Investigator(Kenkyū-buntansha) FUJII Shingo  KYOTO UNIVERSITY, Graduate school of Medicine, Professor, 医学研究科, 教授 (30135579)
FUJITA Jun  KYOTO UNIVERSITY, Graduate school of Medicine, Professor, 医学研究科, 教授 (50173430)
Project Period (FY) 2001 – 2002
Keywordsextravillous trophoblast / cancer invasion / DPP-IV / chemokine / ephrin
Research Abstract

During early placentation, Human trophoblasts differentiate into exfcravillous trophohlasts (EVTs), which invade into maternal uterine tissue inducting walls of maternal spiral arteries to establish effective placental circulation. Although the mechanism of EVT invasion are reported to resemble to that of cancer invasion, invasion of EVT is limited to inner one third of uterine myometrium. We have previously analyzed regulatory mechanisms of EVT invasion, and found that some molecules expressed in EVTs might regulate the invasion of EVTs into maternal tissue. Among molecules which regulate EVT invasion, we have found that CD9 suppressed the invasion of uterine endometrial cancer cell line. This finding suggests that molecules which regulate EVT invasion might also regulate cancer invasion. Therefore in this project, we investigated the roles of molecules expressed in human EVTs on the regulatory mechanisms of cancer invasion. At first, we analyzed the expression of molecules expressed … More in human EVTs in female pelvic organ and in gynecological cancer tissues. By immunohistochemical and Northern blot analyses, Dipeptidyl peptidase IV (DPP-IV), which inhibits EVT invasion in vitro, was expressed in human endometrial epithelial cells of secretory phase. However, DPP-IV expression was decreased in endometrial carcinoma tissues. In addition, both ephrin A1 and its ligand Eph tyrosine kinase type receptor were found to expressed in endometrial cancer tissues. Among these molecules, DPP-IV peptidase activity was found to suppress human choriccarcinoma derived cell line. Subsequent analyses clarified that inactivation of chemokine RANTES is involved in this anti-invasive effect of DPP-IY
In conclusion, some molecules expressed in human EVTs such as DPP-IV or ephrin/Eph system were found to be expressed in normal female pelvic organ and gynecological cancer. Further investigation of these molecules expressed in human EVTs might clarify the regulatory mechanisms of cancer invasion Less

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Higuchi T: "Cyclic AMP enhances the expression of an extravilloustrophoblast marker, melanoma cell adhesion molecule, in choriocarcinoma cellJEG3 and human chorionic villous explant cultures"Mol Hum Reprod. (in press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujiwara H: "Acute elevation of plasma D-dimer levels associated with rupture of an ovarian endometriotic cyst : Case report"Hum Reprod. 18(2). 338-341 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kosaka K: "Human peripheral blood mononudear cells enhance cell-cell interaction between human endometrial epithelial cells and BeWo-cell spheroids"Hum Reprod. 18(1). 19-25 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujiwara H: "Human endometrial epithelial cells express ephrin A1 : possible interaction between human blastocysts and endometrium via Eph-ephrin system"J Clin Endocrinol Metab. 87(12). 5801-5807 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kosaka K: "Human chorionic gonadotropin (HCG) activates monocytes to produce interleukin-8 via a different pathway from luteinizing hormone/HCG receptor system"J Clin Endocrinol Metab. 87(11). 5199-5208 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sato Y: "Involvement of dipeptidyl peptidase IV in extravillous trophoblast invasion and differentiation"J Clin Endocrinol Metab. 87(9). 4287-4296 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Higuchi T. et al.: "Cyclic AMP enhances the expression of an extravillous trophoblst marker, melanoma cell adhesion molecules,in choriocarcinoma cell JEG3 and human chorionic villous exolant cultures"Mol Hum Reprod. (in press). (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujiwara H. et al.: "Acute elevation of plasma D-dimer levels associated with rupture of an ovarian endometriotic cyst: Case report"Human Reproduction. 18. 338-341 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kosaka K. et al.: "Human peripheral blood mononuclear cells enhance cell-cell interaction between human endotnetrial epithelial cells and BeWo-cell spheroids"Human Reproduction. 18. 19-25 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujiwara H. et al.: "Human endometrial epithelial cells express ephrin A1: possible interaction between human blastocysts and endometrium via Eph-ephrin system"J Clin Endocrinol Metab.. 87. 5801-5807 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kosaka K. et al.: "Human chorionic gonadotropin(HOG) activates monocytes to produce interlenkin-8 via a different pathway from luteinizing hormone/HCG receptor system"J Clin Endocrinol Metab.. 87. 5199-5208 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sato Y. et al.: "Involvement of dipeptidyl peptidase IV in extravillcus trophoblast invasion and differentiation"J Clin Endocrinol Metab.. 87. 4287-4296 (2002)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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