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2003 Fiscal Year Final Research Report Summary

The Lole of Local Regulator in the Rat Ovary during Ovulatory Process

Research Project

Project/Area Number 13671722
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionThe University OF Tokushima

Principal Investigator

IRAHARA Minoru  The University of Tokushima, School of Medicine, Professor, 医学部, 教授 (20160070)

Project Period (FY) 2001 – 2003
KeywordsGRO-CINC / CHEMOKINE / INTERLEUKINE / OVULATION / FOLLICULARATRESIA
Research Abstract

A cytokine-induced neutriophil chemoattractant (CINC/gro), which belongs to the interieukin (IL)-8 family, acts as a functional chemoattractant for neutrophils in rats. In the present study, we examined whether CINC/gro contributes to the ovulation process in the rat ovulation system. In rat ovaries, CINC/gro was immunohistochemicaliy recognized in the theca layer of the antral follicle but not in the granulosa cells. To clarify the role of CINC/gro in the ovulation process, CINC/gro protein and mRNA were examined during pregnant mare serum gonadotropin (PMSG)-hCG treatment. CINC/gro protein did not increase as a result of PMSG injection. However, it increased rapidly after hCG injection and peaked at 6 h after hCG.CINC/gro mRNA was also strongly expressed after hCG injection. The increase of CINC/gro protein followed increases in IL 1 p and tumor necrosis factor (TNF). In the whole ovarian dispersate culture, FSH, hCG, IL 1 p, and TNF stimulated the production of CINC/gro protein in a dose-dependent manner. In particular, the stimulatory effects of IL 1β and TNF were stronger than those of gonadotropins. The number of ovulated ooCytes counted under microscopy in the salpinx wash solution was significantly lower in the group which endogenous CINC/gro effect was neutralized by intraperitoneal injection of the antibody to CINC/gro than in the control group. These results suggest that CINC/gro plays an important role in the rat ovulation process by attracting neutrophils. CINC/gro increased just prior to ovulation, and it may be regulated directly by cytokines such as IL 1 Q and TNF and indirectly by gonadotropins

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Toshiyuki Yasui: "Stimulatory effect of the herbal medicine Keishi-bukuryo-gan on a cytokine-induced neutrophil chemoattractant, in rat ovarian cell culture."Am J Reprod Immunol. 50・1. 90-97 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshiyuki Yasui: "The herbal medicine Unkei-to stimulates the secretion of a cytokine-induced neutrophil chemoattractant, CINC/gro, in the rat ovarian cell culture."Am J Reprod Immunol. 49・1. 14-20 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Michiko Tezuka: "Effects of leptin on gonadotropin secretion in juvenile female rat pituitary cells."European Journal of Endocrinology. 146・2. 261-266 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hirokazu Uemura: "Serum osteoprotegerin/osteoclastogenesis-inhibitory factor during pregnancy and lactatio and the relationship with calcium-regulating hormones and bone turnover markers."Journal of Endocrinology. 33・5. 353-359 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mitsuhiro Kimura: "The obesity in bilateral ovariectomized rats is related to a decrease in the expression of leptin receptors in the brain."Biochemical and Biophysical Research Communications. 290・4. 1349-1353 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Minoru Irahara: "Orexin-A suppresses the pulsatile secretion of luteinizing hormone via beta-endorphin."Biochemical and Biophysical Research Communications. 281・1. 232-236 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshiyuki Yasui: "Stimulatory effect of the herbal medicine Keishibukuryo-gan on a cytokine-induced neutrophil chemoattractant, in rat ovarian cell culture"Am J Reprod Immunol.. 50(1). 90-97 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toshiyuki Yasui: "The herbal medicine Unkei-to stimulates the secretion of a cytokine-induced neutrophil chemoattractant, CINC/gro, in the rat ovarian cell culture"Am J Reprod Immunol.. 49(1). 14-20 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Michiko Tezuka: "Effects of leptin on gonadotropin secretion in juvenile female rat pituitary cells"Eur J Endocrinol.. 146(2). 261-266 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirokazu Uemura: "Serum osteoprotegerin/osteoclastogenesis-inhibitory factor during pregnancy and lactation and the relationship with calcium-regulating hormones and bone turnover markers"J Endocrinol.. 174(2). 353-359 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mitsuhiko Kimura: "The obesity in bilateral ovariectomized rats is related to a decrease in the expression of leptin receptors in the brain"Biochem Biophys Res Commun.. 290(4). 1349-1353 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Minoru Irahara: "Orexin-A suppresses the pulsatile secretion of luteinizing hormone via beta-endorphin"Biochem Biophys Res Commun.. 281(1). 232-236 (2001)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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