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2004 Fiscal Year Final Research Report Summary

Cytokine induced changes observed in cerebral vascular reactivity in cerebral microcirculation using intaraviatal microscopy : a new model of periventricular leukomalacia

Research Project

Project/Area Number 13671723
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionEhime University

Principal Investigator

OCHI Hiroshi  Ehime University, University Hospital, Associate Professor, 医学部附属病院, 助教授 (50194569)

Co-Investigator(Kenkyū-buntansha) MATSUBARA Keiichi  Ehime University, University hospital, Lecturer, 医学部附属病院, 講師 (80263937)
TOMIHIRO Katayama  Ehime University, School of Medicine, Instructor, 医学部, 助手 (90304625)
Project Period (FY) 2001 – 2004
KeywordsPeriventricular Leukomalacia / Microcirculation / Cytokine / Angiotensin / Arteriole
Research Abstract

A progressive relationship was noted between increasing TNF-α levels and increased risk of neonatal periventricular leukomalacia. The purpose of this study was to determine the changes in cerebral vascular reactivity to vasoactive substances in the cerebral arteriole of normal rats indeuced by intraperitoneal administration of TNF-α.
Cerebral arterioles were observed by intravital microscopy assisted by a video imager with cranial window method. We determined the cerebral vascular reactivity to norepinephrine (NE) (0.01〜10 μg/kg/min) and angiotensin II (AT II) (0.01〜10 μg/kg/min) in normal rats. Furthermore, we evaluated the changes in cerebral vascular reactivity to AT-II (1μg/kg/min) indeced by intraperitoneal administration of TNF-α (25μg/kg).
The administration of 0.01, 0.1, 1, 10 μg/kg/min NE induced dose-dependent arteriolar vasoconstriction. Maximum vasoconstriction of 29±14% was attained at a concentration of 10 μg/kg/min NE. On the other hand, the administration of 0.01, 0.1, 1, 10 μg/kg/min AT II did not induced significant arteriolar vasoconstriction. A significant change in cerebral vascular reactivity to AP II was observed after intraperitoneal administration of TNF-α. Vasoconstriction of 7±5% in control group was increased to 23±14% in TNF-α administered group (p<0.05).
Our data suggest that cerebral arterial response to AT II is different from the response to NE, and TNF-α modulates cerebral arterial response to AT-II.

  • Research Products

    (8 results)

All 2004 2003

All Journal Article (8 results)

  • [Journal Article] Effects of oxidized low-density lipoprotein on leukocyte-endothelial interactions in the rat mesenteric microcirculation during pregnancy.2004

    • Author(s)
      Katayama T
    • Journal Title

      Am J Obstet Gynecol 191

      Pages: 322-327

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Effects of oxidized low-density lipoprotein on leukocyte-endothelial interactions in the rat mesenteric microcirculation during pregnancy.2004

    • Author(s)
      Katayama T, Tanaka-Shiraishi A, Kiyomura M, Matsumoto T, Kusanagi Y, Ito M.
    • Journal Title

      Am J Obstet Gynecol 191

      Pages: 322-327

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Changes in serum concentrations of tumor necrosis factor alpha and adhesion molecules in normal pregnant women and those with pregnancy-induced hypertension.2003

    • Author(s)
      Matsubara K
    • Journal Title

      J Obstet Gynecol Res 29

      Pages: 422-426

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Clinical significance of normalization of uterine artery pulsatility index with maternal heart rate for the evaluation of uterine circulation in pregnancy-induced hypertension.2003

    • Author(s)
      Ochi H
    • Journal Title

      Ultrasound Obstet Gynecol 21

      Pages: 459-463

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Elevated levels of adhesion molecules derived from leukocytes and endothelial cells in patients with pregnancy-induced hypertension.2003

    • Author(s)
      Abe E
    • Journal Title

      Hypertensive Pregnancy 22

      Pages: 31-43

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Changes in serum concentrations of tumor necrosis factor alpha and adhesion molecules in normal pregnant women and those with pregnancy-induced hypertension.2003

    • Author(s)
      Matsubara K, Abe E, Ochi H, Kusanagi Y, Ito M.
    • Journal Title

      J Obstet Gynaecol Res 29

      Pages: 422-426

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Clinical significance of normalization of uterine artery pulsatility index with maternal heart rate for the evaluation of uterine circulation in pregnancy-induced hypertension.2003

    • Author(s)
      Ochi H, Kusanagi Y, Katayama T, Matsubara K, Ito M.
    • Journal Title

      Ultrasound Obstet Gynecol 21

      Pages: 459-463

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Elevated levels of adhesion molecules derived from leukocytes and endothelial cells in patients with pregnancy-induced hypertension.2003

    • Author(s)
      Abe E, Matsubara K, Ochi H, Ito M, Oka K, Kameda K.
    • Journal Title

      Hypertens Pregnancy 22

      Pages: 31-43

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2006-07-11  

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