2002 Fiscal Year Final Research Report Summary
Role of macrophages in bone destruction in cholesteatoma otitis media
| Project/Area Number |
13671811
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Hyogo College of Medicine |
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Principal Investigator |
SAKAGAMI Masafumi Hyogo College of Medicine Professor, 医学部, 教授 (10170573)
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| Co-Investigator(Kenkyū-buntansha) |
TSUJIMURA Toru Hyogo College of Medicine Associate Professor, 医学部, 助教授 (20227408)
SEO Toru Hyogo College of Medicine Assistant Professor, 医学部, 講師 (30258149)
TSUI Kojiro Hyogo College of Medicine Research Associate, 医学部, 助手 (30330450)
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| Project Period (FY) |
2001 – 2002
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| Keywords | macrophage / osteoclast / cholesteatoma otitis media / catepsin K / TRAP / osteopontin / cytokine / tympanoplasty |
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| Research Abstract |
Many studies reported that the typical osteoclasts are infrequent in human chlesteatoma biopsies. We previously reported that many macrophages were found in subepithelial tissue under cholesteatoma matrix. Therefore, we hypothesized that the macrophage has an activity of the osteoclast-like cell. Under informed consent to the patients, cholesteatoma matrix, subepithelial granulation tissue and ossicles adjacent to matrix were taken out for specimens during tympanoplasty.
Results were the followings ; 1) many multinuclear cells were stained in subepithelial connective tissue with immunohistochemistry of CD68 antibody. 2) Cathepsin K and TRAP (tartrate-resistant acid phosphatase), which are usually expressed in osteoclasts, were observed in macrophages with immunohistochemistry and in situ hybridization. 3) Cathepsin K and TRAP were also recognized by RT-PCR. 4) OPN (osteopontin), which is necessary to attach to the surface of osteoclasts, was observed in macrophages with immunohistochemistry and in situ hybridization. 5) Cytokines of cholesteatoma matrix and granulation tissue (IL-1, IL-6, TNF-a, M-SCP) were expressed in the culture liquid.
These findings suggested that inflammatory cytokines induced macrophages from the bone marrow to subepithelial connective tissue and that they became osteoclast-like macrophages to attach to the bone surface.
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