Development of improving method for respiratory function in newborn diaphragma hernia

2002 Fiscal Year Final Research Report Summary

• Project/Area Number: 13671863
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatric surgery
• Research Institution: YAMAGATA UNIVERSITY
• Principal Investigator: YAMAGIWA Iwao Yamagata Univ, Med, Assistant Professor, 医学部, 助教授 (60182424)
• Co-Investigator(Kenkyū-buntansha): SHIMAZAKI Yasuhisa Yamagata Univ, Med, Professor, 医学部, 教授 (60116043) ; INUI Kiyoshige Yamagata Univ, Med, Assistant Professor, 医学部, 講師 (70250941)
• Project Period (FY): 2001 – 2002
• Keywords: diaphragma hernia / KGF / cell transplantation

Research Abstract

Animal model of diaphragmatic hernia was introduced by administration of nitrofen. Type II alveolar cell was transfected with plasmid encoding HGF, or KGF gene by lipofection method. The efficacy of transfection was 10-20%. The excretion of introduced proteins from cells in the culture was recognized by SDS PAGE. Rats were anestetized by pentobarbital and intratracheal intubarion was carried out. 1 x 10^<-6> cells transfected with specific genes were injected from periferal veins. These cells were trapped in the microcirculation of lung tissue. Cells were survived in the lung tissue. Rats of diaphragmatic hernia model were used for studying the efficacy of the cell transplantation from peripheral vein. KGF transfected cells improved the saturation of rats after injection from the veins.