2002 Fiscal Year Final Research Report Summary
ANALYSIS OF EFFECTS OF MECHANICAL STRESS ON APOPTOSIS OF KELOID DERIVED FIBROBLASTS
| Project/Area Number |
13671876
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | CHIBA UNIVERSITY |
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Principal Investigator |
YOSHIMOTO Shinya Chiba University, Graduate School of Medicine, Associate Professor, 大学院・医学研究院, 助教授 (90220748)
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| Co-Investigator(Kenkyū-buntansha) |
NOMURA Jun Chiba University, Department of Environmental Medicine, Associate Professor, 教育学部, 助教授 (30252886)
KITA Kazuko Chiba University, Graduate School of Medicine, Lecturer, 大学院・医学研究院, 講師 (80302545)
ICHINOSE Masaharu Chiba University, Graduate School of Medicine, Professor, 大学院・医学研究院, 教授 (90082156)
SUGITA Katsuo Chiba University, Department of Medicine, Professor, 教育学部, 教授 (40211304)
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| Project Period (FY) |
2001 – 2002
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| Keywords | keloid / fibroblasts / apoptosis / p53 / Bax / MAP Kinase / p38 / Mechanical stress |
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| Research Abstract |
Keloids are characterized by the proliferation of fibroblasts and increased accumulation of extracellular matrix. Excessive growth of fibroblasts and lower rate of apoptosis has been implicated in the etiology of keloids. Malfunction of apoptotic signaling pathways are thought to be one of mechanisms underlying tumorigenic growth of keloid scar. To investigate cellular signaling factors responsible for the malfunction, antimetabolic agents were utilized as an inducer of unusual response in keloid-derived cells. Fibroblasts from keloid skin (KF) and those from normal skin (NF) were compared with respect to their sensitivity to the anti-proliferative effects of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). In KF and NF cells, enhanced levels of p53 and Bax amounts were observed after the treatment. Moreover, human fibroblasts were highly susceptible to apoptosis after mechanical stress exposure by a free-fall. The expression of nuclear p53 and cytoplasmic Bax in the fibroblasts increased at 4 h after mechanical stress exposure. Thus, these molecules may contribute cytotoxic effects of MNNG in KF and NF.
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