• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2002 Fiscal Year Final Research Report Summary

Induction of differentiate into odontogenic cells from murine embryonic stem cell

Research Project

Project/Area Number 13671900
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionTottori University

Principal Investigator

YAMAZAKI Hidetoshi  Tottori University, Faculty of Medicine, Associate Professor, 医学部, 講師 (00283987)

Project Period (FY) 2001 – 2002
KeywordsNeural crest / Embryonic stem cell / Regeneration of tooth / In virto culture system / Odontoblast / Mouse / Osteoclast / Osteoblast
Research Abstract

We have found that odontoblasts are derived from neural crest cells using P0-cre mice and Rosa26R mice. In these mice, neural crest cells are easily detected as LacZ+ cells and these mice enable us to isolate LacZ+ neural crest cells in vivo. Furthermore, we have found that BMP signalings are important for molar cusp formation in tooth molar organ culture. We also developed the culture system of embryonic stem cells to induce the differentiation of osteoclasts and osteoblasts. The results are shown as follows.
1) Using P0-cre mice and Rosa26R mice, neural crest cells and their progeny can be easily detected as LacZ+ cells and can be isolated. Multipotent neural crest cells with a potential of differentiate into melanocyte and neuroal cells were present in the fetal thymus.
2) Using soluble formed BMP type I receptor to inhibit BMP signaling in tooth organ culture, the inhibition of BMP signaling at both E13.5 and E16.5 tooth buds leads to abnormal cusp formation. These results indicate that BMP signaling is essential for the proper cusp formation in all stags of tooth morphogenesis.
3) We developed the culture system of embryonic stem cells to induce the differentiation of osteoclasts and osteoblasts.
We have already published the papers 3) and the manuscripts 1) 2) have been submitted. Recently, we established the culture system of melanocytes, which are derived from neural crest cells are induced from murine embryonic stem cells. Furthermore, we also established the Dsp-LacZ mice, which expressed LacZ in odontoblast specially. Therefore, we may establish new system of differentiation into odontoblast from embryonic stem cell through neural crest cells.

  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Yamada, T: "Regulation of osteoclast development by Notch signaling directed to osteoclast precursors and through stromal cells"Blood. 101. 2227-2234 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshino, M: "Reduction of osteclasts in the critical embryonic period is essential for inhibition of mouse tooth eruption"J.Bone.Miner.Res.. 18. 108-116 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sugaya, T: "Clonotypic Analysis of T Cells Accumulating at Arthritic Lesions in HTLV-I env-pX Transgenetic Rats"Exp Mol Pathol.. 72(1). 56-61 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nagai, Y: "Requirement for MD-1 in cell surface expression of RP105/CD180 and B cell responsiveness to lipopolysaccharide"Blood. 99. 1699-1705 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hemmi, H: "Temporal and spatial localization of osteoclasts in colonies from embryonic stem cells"Biochem Biophys Res Commun.. 280. 526-534 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamazaki, H: "Presence of osteoclast precursors in colonies cloned in the presence of hematopoietic colony-stimulating factors"Exp Hematol.. 29. 68-76 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsuneto, M: "Methods in Enzymology"In vitro differentiation of mouse ES cells into hematopoietic, endothelial, and osteoblastic cell lineages : A Possibility of in vitro organogenesis (in press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamane,Y.,et al.: "Wnt signaling regulates hemopoiesis through stromal cells"J.Immunol. 167. 765-772 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okuyama,H.,et sl.: "Development of osteoclast lineage cells"Research Advances in Blood. (Global Research Network). 1. 75-84 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakamaru,Y., et al.: "Immunological hyperresponsiveness in HTLV-ILTR-env-pX transgenic rats: a prototype animal model for collagen vascular and HTLV-I-related inflammatory diseases"Pathobiology. 69. 11-18 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kunisada,T., et al.: "Ligands for receptor tyrosine kinases expressed in the skin as environmental factors for melanocyte development"J. Invest. Dermatol. Symp. Proc.. 6. 6-9 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hemmi,H., et al.: "Skin antigens in the steady state are trafficked to regional lymph nodes by transforming growth factor-betal-dependent cells"Int Immunol. 13. 695-704 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamazaki,H., et al.: "Presence of osteoclast precursors in colonies cloned in the presence of hematopoietic colony-stimulating factors"Exp Hematol. 29. 68-76 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hemmi,H., et al.: "Temporal and spatial localization of osteoclasts in colonies from embryonic stem cell"Biochem Biophys Res Commun. 280. 526-534 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hayashi,S., et al.: "In vitro induction of differentiation from embryonic stem cells: looking forward to regenerative medicine"Nihon Hnsenbyo Gakkai Zasshi. 70. 121-126 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagai,Y., et al.: "Requirement for MD-1 in cell surface expression of RP105/CD180 and B cell responsiveness to lipopolysaccharide"Blood. 99. 1699-1705 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sugay,T., et al.: "Clonotypic Analysis of T Cells Accumulating at Arthritic Lesions in HTLV-1 env-pX Transgenic Rats"Exp. Mol. Pathol.. 72. 56-61 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamada,T., et al.: "Regulation of osteoclast development by Notch signaling directed to osteoclast precursors and through stromal cells"Blood. 101. 2227-2234 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshino,M., et al.: "Reduction of osteoclasts in the critical embryonic period is essential for inhibition of mouse tooth eruption"J. Bone. Miner. Res.. 18. 108-116 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsuneto,T., et al., Eds. P.M. Wassaraman, G.M.Keller: "In vitro differentiation of mouse ES cells into hematopoietic, endothelial, and osteoblastic cell lineages: A possibility of in vitro organogenesis, In differentiation of Embryonic Stem Cells, Methods in Enzymology"Academic Press.

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2004-04-14  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi