2002 Fiscal Year Final Research Report Summary
Overexpression of antimicrobial peptides in cells makes them resistant bacterial infection
| Project/Area Number |
13671907
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Health Sciences University of Hokkaido |
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Principal Investigator |
KAKU Toru Health Sciences University of Hokkaido, School of Dentistry, Professor, 歯学部, 教授 (60133253)
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| Co-Investigator(Kenkyū-buntansha) |
ARAKAWA Toshiya Health Sciences University of Hokkaido, School of Dentistry, Assistant Professor, 歯学部, 講師 (40306254)
NAKASHIMA Keisuke Health Sciences University of Hokkaido, School of Dentistry, Associate Professor, 歯学部, 助教授 (80227785)
ABIKO Yoshihiro Health Sciences University of Hokkaido, School of Dentistry, Associate Professor, 歯学部, 助教授 (90260819)
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| Project Period (FY) |
2001 – 2002
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| Keywords | Defensin / Transfection / Bacterial infection / Periodontitis / Macrophage |
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| Research Abstract |
Antimicrobial peptides such as β defensins, LL-37, hCAP-18 are involved in defensive function of oral epithelium. It is still unclear how strong protective effect of the peptides on the bacterial infection. The present study examined whether overexpression of the peptides in macrophage cell lines, J774.1, made them resistance bacterial infection.
Two vectors showing β defensin-1 (hBD-1) and -2 (hBD-2) expressions were transfected into J774.1 cell lines by lipofection method. Overexpressions of hBD-1 and -2 in J774.1 cell lines were confirmed by RT-PCR and western blot methods. The cell lines hBDs overexpressed (hBD-1/J774.1, hBD-2/J774.1) and non transfected cell line were used. For bacterial infection in vitro, Actinobacillus actinomycetemcomitance (Aa) was used. The bacterial infections were evaluated as cell death of the cell lines by standard quantitative, MTT and DNA fragmentation assays. The ration of cell death in hBD-1/J774.1 or hBD-2/J774.1 is less than that in J777.1. The results indicate that the overexpression of hBDs make them resistant bacterial infection.
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[Publications] Abiko, Y, Suraweera, AK, Nishimura, M, Arakawa, T, Takuma, T, Mizoguchi, I, Kaku, T: "Differential expression of human beta-defensin 2 in keratinized and non-keratinized oral epithelial lesions; immunohistochemistry and in situ hybridization"Virchows Arch.. 438. 248-253 (2001)
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[Publications] Liu, F, Abiko, Y, Nishimura, M, Kusano, K, Shi, S, Kaku, T: "Expression of inflammatory cytokines and beta-defensin 1 mRNAs in porcine epithelial rests of Malassez in vitro"Med Electron Microse.. 34. 174-178 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Abiko, Y, Murata, M, Ito, Y, Taira, T, Nishimura, M, Arisue, M, Inoue, T, Shimono, M, Kuboki, Y, Kaku, T: "Immunohistochemical localization of amelogenin in human odontogenic tumors, using a polyclonal antibody against bovine amelogenin"Med Electron Microsc.. 34. 185-189 (2001)
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[Publications] Abiko, Y, Jinbu, Y, Noguchi, T, Nishimura, M, Kusano, K, Amaratumga, P, Shibata, T, Kaku, T: "Upregulation of human beta-defensin 2 peptide expression in oral lichen planus, leukoplakia and candidiasis"Pathology. 198. 537-542 (2002)
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「研究成果報告書概要(欧文)」より
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[Publications] Abiko, Y, Nishimura, M, Kusano, K, Nakashima, K, Okumura, K, Arakawa, T, Takuma, T, Mizoguchi, I, Kaku, T: "Expression of MIP-3Eo/CCL20, a macrophage inflammatory protein in oral squamous cell carcinoma"Archives of Oral Biol.. 48. 171-175 (2003)
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[Publications] Abiko, Y, Nishimura, M, Kusano, K, Yamazaki, M, Arakawa, T, Takuma, T, Kaku, T: "Upregulated expression of human E* defensin-1 and -3 mRNA differentiation of keratinocyte immortalized cell lines, HaCaT and PHK16-0b"Journal Dermatol Science. 31. 225-228 (2003)
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