2002 Fiscal Year Final Research Report Summary
A Study on development mechanism of Odontogenic Tumor
| Project/Area Number |
13671927
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Aichi-Gakuin University School of Dentistry |
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Principal Investigator |
KAMEYAMA Yoichiro Aichigakuin University, School of Dentistry, Department of Pathology, Professor, 歯学部, 教授 (70113066)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Emiko Aichigakuin University, School of Dentistry, Department of Patholog, Lecturer, 歯学部, 講師 (60111001)
MAEDA Hatsuhiko Aichigakuin University, School of Dentistry, Department of Patholog, Associate Professor, 歯学部, 助教授 (30175591)
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| Project Period (FY) |
2001 – 2002
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| Keywords | animal / jaw bone / tooth germ / organ culture / heterocyclic amine / growth factor |
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| Research Abstract |
To investigate the pathological mechanism of the development of odontogenic cyst and odontogenic tumor, the effects of carcinogens, Trp-P-2 and MeIQx, and growth factor TGF-α on the mouse tooth-germ in vitro and in vivo. In experiment I, effects of Trp-P-2 and TGF-α , alone and in combination, on the cell proliferation and differentiation of the organ-cultured molar tooth-germ of ddY embryonic mouse were studied histologically and immunohisochemically (PCNA). TGF-α increased the cell proliferation of the organ-cultured tooth-germ, but inhibited its differentiation. Trp-P-2 inhibited the cell proliferation of the organ-cultured tooth-germ. In experiment II, effects of MeIQx and TGF-α, alone and in combination, on the cell proliferation and differentiation of the mouse molar tooth-germs, which were transplanted into BALB/C nude mouse following organ-culture. Both of MeIQx and TGF-α increased the cell proliferation of the transplanted mouse tooth-germ. In experiment III, effects of Trp-P-2 and TGF-α, alone and in combination, on the cell proliferation and differentiation of the mouse molar tooth-germs, which were transplanted into BALB/C nude mouse following organ-culture. Trp-P-2 in combination with TGF-α increased the cell proliferation of the odontogenic epithelium of the transplanted tooth-germs, which showed keratinization and cyst formation. The results of the present study may suggest that some carcinogens and growth factor are involved in the development of odontogenic cyst and odontogenic tumor.
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