2003 Fiscal Year Final Research Report Summary
Inhibitory effect of human α_2-plasmin inhibitor and it's intensified compound by S-nitrosylation on the activity of arginine-specific cysteine proteinase from Porphyromonas gingivalis.
| Project/Area Number |
13671931
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Fukuoka Dental College |
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Principal Investigator |
KAMINISHI Hidenori Fukuoka Dental College, Infection Biology, Professor, 歯学部, 教授 (90084300)
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| Co-Investigator(Kenkyū-buntansha) |
AKAIKE Takaaki Kumamoto University, Medical Microbiology, Professor, 医学部, 助教授 (20231798)
TAKEUCHI Hisako Fukuoka Dental College, Infection Biology, Research Assistant, 歯学部, 助手 (50198800)
OGURA Rieko Fukuoka Dental College, Infection Biology, Research Assistant, 歯学部, 助手 (80320331)
HAMAMOTO Takayoshi The Chemo-Sero-Therapeutic Research Institute, Research Fellow, 研究員
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| Project Period (FY) |
2001 – 2003
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| Keywords | P. gingivalis / gingipain / α_2-plasmin inhibitor / S-nitrosylation / α2アンチプラスミン / α1プロテアーゼインヒビター / ベジクル |
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| Research Abstract |
We investigated the effect of human α_2-plasmin inhibitor (α_2PI) and S-nitrosylated α_2-plasmin inhibitor (S-NO-α_2PI) on the activity of arginine-specific cysteine proteinase (gingipain, RGP) from Porphyromonas gingivalis, an aetiological bacterium of adult periodontitis, with the use of fluorogenic substrates. We discovered that human α_2PI as well as S-NO-α_2PI play a inhibitory effect to the RGP in a dose-dependent manner. The α_2PI, one of the members of serpin family, is well known as a specific inhibitor of plasmin, a typical serine proteinase. However, few studies have been reported concerning the inhibitory potency of humoral factors, such as α_1-protease inhibitor or α_2-macroglobulin, against cysteine proteinases especially RGP. In this study, we found a novel function of α_2PI as a potent inhibitor of RGP. The inhibitory potencies of the S-nitrosylated derivative against both human plasmin and RGP were slightly higher than those of the intact α_2PI. These results strongly suggest that S-NO-α_2PI is a useful tool for preventing the virulence of P. gingivalis.
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