2002 Fiscal Year Final Research Report Summary
Cell Death and Cell Proliferation of Myoepithelial cells in Duct-Ligated Submandibular Glands
| Project/Area Number |
13671961
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
病態科学系歯学(含放射線系歯学)
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
TAKAHASHI Shigeru Hokkaido Univ., Grad. School of Dent., Inst., 大学院・歯学研究科, 助手 (70241338)
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| Co-Investigator(Kenkyū-buntansha) |
DOMON Takanori Hokkaido Univ., Grad. School of Dent., Inst., 大学院・歯学研究科, 助手 (50217618)
YAMAMOTO Tsuneyuki Hokkaido Univ. Grad. School of Dent., Asso. Prof., 大学院・歯学研究科, 助教授 (80200822)
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| Project Period (FY) |
2001 – 2002
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| Keywords | Submandibular Gland / Myoepithelial Cell / Apoptosis / Cell Proliferation |
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| Research Abstract |
This study was designed to determine whether apoptosis and proliferation of myoepithelial cells occur in atrophic rat submandibular glands. The excretory duct of the right submandibular gland was doubly ligated with the metal clips. The atrophic right submandibular glands removed after 1-28 days of duct-ligation were investigated using immunohistochemical double staining for actin as a marker for myoepithelial cells and proliferating cell nuclear antigen (PCNA), double staining for actin immunohistochemistry, nick-end-labeling (TUNEL) as a marker for apoptotic cells, and transmission electron microscopy (TEM). A few PCNA- and no TUNEL-positive myoepithelial cells were found in the control submandibular glands taken from animals with no operation. In the experimental glands, PCNA-positive myoepithelial cells were common 2 and 3 days after duct ligation and then decreased in number. TUNEL-positive myoepithelial cells appeared at 2 days and were also identified by TEM. These observations suggest that both apoptosis and proliferation of myoepithelial cells occur, especially in the early phase of atrophy, in the rat submandibular gland.
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