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2003 Fiscal Year Final Research Report Summary

Construction of the custom-made DNA chip for pathogenic diagnosis of bacteria related with periodontal disease

Research Project

Project/Area Number 13671981
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 病態科学系歯学(含放射線系歯学)
Research InstitutionNihon University

Principal Investigator

HIRATSUKA Koichi  Nihon University, School of Dentistry at Matsudo, Lecturer (Full-Time), 松戸歯学部, 講師 (80246917)

Project Period (FY) 2001 – 2003
Keywordsperiodontal disease / pathogen / microarray / transcriptome / examination / environment / Porphyromonas gingivalis
Research Abstract

It takes long time and much cost to analyze the expressions of all known-functional genes by the conventional method. Furthermore, we cannot expect to utilize any commercial-based microarrays for oral bacterial in the future. Therefore, we prepared a DNA custom-made array of pathogenicity-associated genes in Porphyromonas gingivalis, strongly associated with disease activity in adult periodontal disease, and established the microarray procedure such as hybridization, wash, and labeling condition, and how to analyze. Consequently, mRNA was good start material for catching low signal intensities of gene expressions, compares with Total RNA. Moreover, it became clear to match results between microarray and Real-time PCR when we used fixed quantity of the labeled external standards from human genes for chip-chip normalization rather than 16S rRNA (internal standard). Following various environmental change was stressed to P. gingivalis and the transcriptome profiling was analyzed (1. Gene expression profiling during growth; 2. Gene expression profiling under hemin limitation; 3. Gene expression profiling under oxidization stress). These results suggested that we can expect not only to detect gene expressions of various pathogen but also to demonstrate the relationship among the pathogenic genes in P. gingivalis. Moreover, this technology expected a possibility that it could monitor of the degree of activity of the bacteria in a periodontal pocket.

  • Research Products

    (2 results)

All Other

All Publications (2 results)

  • [Publications] 平塚 浩一: "Porphyromonas gingivalis gingipain遺伝子マイクロアレイの開発"医学と生物学. 144. 133-137 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Hiratsuka, M.Araki, M.Erna, Y.Abiko: "Development of DNA microarray for Porphyromonas gingivalis gingipain genes."Med.Biol.. 144(4). 133-137 (2002)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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