2002 Fiscal Year Final Research Report Summary
Risk assessment of root canal disinfectants in terms of endocrine-disruption and development of their substitutes
| Project/Area Number |
13671998
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
TARUMI Hisashi Osaka University Grad. Sch. of Dentistry, Research Associate, 大学院・歯学研究科, 助手 (00294108)
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| Co-Investigator(Kenkyū-buntansha) |
KINOMOTO Yoshifumi Osaka University Dental Hospital, Assistant Professor, 歯学部附属病院, 講師 (10252694)
EBISU Shigeyuki Osaka University Grad. Sch. of Dentisrry, Professor, 大学院・歯学研究科, 教授 (50116000)
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| Project Period (FY) |
2001 – 2002
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| Keywords | Root canal disinfectant / endocrine-disrupting chemicals / environmental hormone / estlvgenicity / reporter gene assay / uterotrophic assay / in vitro / in vivo |
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| Research Abstract |
In this study, estrogenic activities of four root canal disinfectants; Formalin/Cresol (FC), Formalin/Guaiacol (FG), p-Chlorophenol/Guaiacol (Methocol^【R!○】), and Guaiacol (Creodon^【R!○】), and their components were investigated in vitro and in vivo.
By in vitro reporter gene assay, Methocol was found to demonstrate estrogenic activity at 10μg/mL or greater, and p-Chlorophenol and p-Cresol were estrogenic at 50 μM and over. For FC and FG, the activity could not be determined as the cells were killed due to the toxicity of the specimens even at the least concentration tested. The estrogenicity shown by Methocol is considered to be dependent upon p-Chlorophenol, and it is possible that FC exhibits estrogenic activity as it contains p-Cresol.
In vivo estrogenicities of the four disinfectants and the components were studied by uterotrophic assay using ovariectomized rats. Disinfectants were administered at 15 μL/kg/day and, the componentsat 50 mg/kg/day. For any of the specimens, no change in the weight or morphology of ovary was observed, indicating that these chemicals were not estrogenic in vivo.
These results indicate that estrogenicities of root canal disinfectants tested were negligible under in vivo condition, although some of their components showed estrogenic-activity by in vitro assays.
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