2002 Fiscal Year Final Research Report Summary

Development of a new sterilization method using ozonated water for the infected carious dentin, pulp, and root canal

Research Project

Project/Area Number	13672005
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Conservative dentistry
Research Institution	Kyushu Dental College
Principal Investigator	TERASHITA Masamichi Kyushu Dental College School of Dentistry Professor, 歯学部, 教授 (00118085)
Co-Investigator(Kenkyū-buntansha)	FUKUIZUMI Takaki Kyushu Dental College School of Dentistry Assistant professor, 歯学部, 講師 (50275442) KITAMURA Chiaki Kyushu Dental College School of Dentistry Research associate, 歯学部, 助手 (50265005)
Project Period (FY)	2001 – 2002
Keywords	Ozonated water / Antimicrobial activity / Infected root canal / Disinfectants / Cytotoxicity / Safety
Research Abstract	Ozone is a strong antimicrobial agent against bacteria, fungi, protozoa and viruses. In this project, we examined the sterilization ability of ozonated water on a experimental model for the infected root canal. After exposure to ozonated water (0.5 mg/L) for 10-30 s, the viability of Enterococcus faecalis, one of the predominant bacteria in a infected root canal, significantly decreased. With ozonated water treatment on a experimental model for the infected root canal using bovine tooth, the number of living Enterococcus faecalis was decreased to one tens compared to that with distilled water, and almost no bacteria were detected after being treated with ozonated water (4 mg/L) for 120 s. There were no significant differences in antimicrobial activity and cytotoxicity to human cell line between ozonated water and oral disinfectants such as povidone iodine and benzethonium chloride. After ozonated water (4 mg/L) treatment for 120 s, antimicrobial effect against mixed infection model for the infected root canal was very high level, even in a deep area of the dentinal tubules. Taken together, these results suggest that ozonated water should be useful in the sterilization for infected root canal.