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2002 Fiscal Year Final Research Report Summary

A study on the developing process of radicular cysts

Research Project

Project/Area Number 13672012
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Conservative dentistry
Research InstitutionNihon University

Principal Investigator

TSURUMACHI Tamotsu  Nihon-University, School of Dentistry, Assistant Professor, 歯学部, 講師 (60139201)

Project Period (FY) 2001 – 2002
KeywordsRadicular cyst / Osmotic pressure / Protein degrading enzyme
Research Abstract

Development of radicular cysts through hydrostatic pressure could be induced by an increase in the osmotic pressure of the confined cyst fluid. Additionally, the cyst may expand due to the actions of cell proliferations and the growth factors. An increased osmolarity would favor increased fluid flow into the cystic cavity generating an increased pressure on the bony cavity walls. This higher pressure would cause additional osteoclastic activity and enlargement of the defect. However, the exact mechanism of increasing osmotic pressure in the cyst cavity has not yet been adequately clarified.
The aim of this study was to investigate the effect of enzyme digestion of proteins or mucopolysaccharides in the cyst fluid on the osmotic pressure which may accelerate radicular cyst development by its hydrostatic pressure, and also to examine the presence of proteinases in cyst fluid, which might contribute to an increase of osmotic pressure by degrading serum proteins.
Osmotic pressure of various concentrations of protein solution (albumin, gelatin and transferring) and hyaluronic acid solution was determined ; these substances are selected as typical endogenous materials of the cyst. Functional activity and molecular weight of proteinases in the cyst fluids were monitored by zymography using casein, serum albumin, transferring, or globulin as a substrate.
The results were as follows :
1. The increase in osmotic pressure in the cyst fluid is mainly due to enzymatic degradation of accumulated proteins, and accumulation of the mucopolysaccharide itself into the cyst fluid.
2. Chymotrypsin-like enzyme is present in the cyst fluids, and that it is similar to stratum corneum chymotryptic enzyme in terms of molecular weight, substrate specificity, effect of inhibitors, and ability to degrade a synthetic substrate.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] 野崎弘晃: "PGE_2が歯根嚢胞由来細胞のPGE_2産生におよぼす影響"日本歯科保存学雑誌. 45. 273-281 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 鶴町 保: "破折器具除去の外科的歯内療法にOrtho-CTを活用した1症例"日本歯科保存学雑誌. 45. 696-701 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 野崎弘晃: "PGE_2が歯根嚢胞由来細胞の骨吸収性サイトカイン産生に及ぼす影響"日本歯科保存学雑誌. 44. 23 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Kurokawa: "Gene expression of LPS receptors in periapical periodontitis"Journal of Dental Research. 80. 768 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 黒川輝将: "根尖性歯周炎形成におけるオステオポンチンの発現について"日本歯科保存学雑誌. 45. 170 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nozaki H: "Effects of prostaglandin E2 (PGE2) on PGE2 production, by radicular cyst-derived cells"The Japanese Journal of Conservative Dentistry. Vol.45-2. 273-281 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsurumachi T: "Clinical application of a small computed tomography for dental use (Ortho-CT) in endodontic surgery : A case report of fractured instruments"The Japanese Journal of Conservative Dentistry. Vol.45-4. 696-701 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nozaki H: "Effect of prostaglandin E2 (PGE2) on bone-resorbing cytokine production by cyst-derived fibroblast- like cells"The Japanese Journal of Conservative Dentistry. Vol.44-spring issue. 23 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kurokawa T: "Gene expression of LPS receptors in periapical periodontitis"Journal of Dental Research. Vol.80-special issue. 768 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kurokawa T: "Gene expression of osteopontin in formation of apical periodontitis"The Japanese Journal of Conservative Dentistry. Vol.45-autum issue. 170 (2002)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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