2002 Fiscal Year Final Research Report Summary
Development of functionally graded DNA films containing cytokines eliciting bone formation
| Project/Area Number |
13672070
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
補綴理工系歯学
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| Research Institution | Fukuoka Dental College |
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Principal Investigator |
FUKUSHIMA Tadao Fukuoka Dental College, Dental Materials, Associate Professor, 歯学部, 助教授 (80084250)
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| Co-Investigator(Kenkyū-buntansha) |
HAYAKAWA Toru Nihon University, School of Dentistry at Matsudo, Dental Materials, Assistant Professor, 松戸歯学部, 講師 (40172994)
OKAHATA Yoshio Tokyo Institute of Technology, Biomolecular Engineering, Professor, 生命理工学部, 教授 (80038017)
INOUE Yusuke Fukuoka Dental College, Dental Hygiene, Associate Professor, 歯科衛生学科, 助教授 (20105688)
KAWAI Tatsushi Aichi-Gakuin University, School of Dentistry, Dental Materials, Professor, 歯学部, 教授 (60167351)
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| Project Period (FY) |
2001 – 2002
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| Keywords | DNA / Lipid / Cytokine / DNA film / DNA-lipid complex / Antibacterial activity / Intercalation / Tensile strength |
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| Research Abstract |
The DNA-lipid complexes with and without cytokines BMP and b-FGF were prepared synthetic lipids from the reaction of n-alkyl alcohol (C=4〜C=20) and amino acids (glycine, L-alanine and L-glutamic acids) and the DNA films were obtained by casting the DNA -lipid complexes from chloroform/ethanol solution. The tensile strengths and intercalation behavior of DNA films were investigated. Antibacterials characteristics of DNA-lipid complexes against bacteria (P. gingivalis, S. mutans, S. aureus and P. aeruginosa) were also investigated. Additionally, effect of the DNA -lipid complexes with cytokines BMP and b-FGF on bone formation was evaluated. The DNA-lipid films had tensile strengths of approximately 2MPa, and formed intercalation complexes with ethidium bromide. The formation of the intercalation complex of DNA-lipid film with ethidium bromide was influenced by the length of the alkyl chains of the synthesized lipids. The complexes prepared from DNA with glycine decyl ester or glycine dodecyl ester, and with L-alanine decyl ester or glycine dodecyl ester showed antibacterial activity. However, the DNA-lipid complexes with cytokines BMP and b-FGF did not elicit bone formation.
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