2002 Fiscal Year Final Research Report Summary
Defense system in the intraorally transplanted skin : the relation between dendritic cells and chemokine system
| Project/Area Number |
13672078
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Surgical dentistry
|
|---|
| Research Institution | HAMAMATSU UNIVERSITY SCHOOL OF MEDICINE (2002)
Tohoku University (2001) |
|---|
Principal Investigator |
FUMINORI Katou HAMAMATSU UNIVERSITY SCHOOL OF MEDICINE, School of Medicine, Associate Professor (60204492)
|
|---|
| Project Period (FY) |
2001 – 2002
|
| Keywords | Oral / Transplanted skin / Chemokine / Dendritic cell |
|---|
| Research Abstract |
Macrophage-derived chemokine (MDC/CCL22) is chemokine ligand for its specific receptor CC chemokine receptor 4 (CCR4) and another chemokine ligand, CCL19 (EBI1 ligand chemokine ; ELC), is the shared receptor for CCR7. We investigated the expression of wo chemokin systems, CCL22/CCR4 and CCL19/CCR7 in the intraorally transplanted skin and lymph node to know the dynamics in the defense system of the intraorally transplanted skin.
Reverse transcriptase-polymerase chain reaction analysis revealed mRNA expression for MDC and CCR4 in the inflamed skin and neck lymph nodes (LNs), but not in normal skin. Meanwhile, RT-PCR analysis revealed the expression of CCL19 and CCR7 in LNs, but not in the inflamed skin. Immunohistochemically, MDC+ cells and CCR4+ cells were located both in the dermis of inflamed skin and the T cell area of LNs. MDC+ cells were identified to be DCs both in inflamed skin and LNs. The majority of CCR4+ cells were CD4+ T cells, accounting for approximately one-third of total CD4+ T cells in the inflamed skin. The majority of DC-Lamp(+) mature DCs in the T-cell area of LNs expressed CCL19 and were surrounded by CCR7(+) lymphocytes. In contast, the majority of DC-Lamp(+) mature DCs in inflamed skin were totally negative for CCL19 and were surrounded by CCR7(-) T cells.
The present study revealed a difference in the function of mature DCs between LNs and chronically inflamed skin.
|
