2003 Fiscal Year Final Research Report Summary
Study for establishing order-made treatment in oral disease.
| Project/Area Number |
13672103
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Kyushu University |
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Principal Investigator |
MORIFUJI Masayo Kyushu University, Dental Hospital, Asistant Professor, 歯学部附属病院, 助手 (90271113)
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| Co-Investigator(Kenkyū-buntansha) |
TASHIRO Kosuke Kyushu University, Faculty of Agriculture, Associate Professor, 農学研究院, 助教授 (00192170)
OHISH Masamichi Kyushu University, Faculty of Dental Sicence, Professor, 歯学研究院, 教授 (70037505)
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| Project Period (FY) |
2001 – 2003
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| Keywords | oral disease / cancer / invasion / metastasis / microarray / order-made treatment |
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| Research Abstract |
Head and neck squamous cell carcinoma (HNSCC) is the sixth most common cancer among men in the development world. The major cause of death from cancer is dissemination of the primary tumor, leading to formation of metastases that are resistant to conventional chemotherapy. The cDNA microarray is a powerful tool for identifying genes that may be applicable for development of novel diagnostic markers and molecular targets for therapeutic purposes. So we made cDNA microarry obtained 9729 arrays. In addition, by orthotopic. implantation into nude mice we investigated metastatic ability in several scc cell lines contained newly established gingival scc cell line. We used this cDNA microarray slide and compared the mRNA levels of nonmetastaticoral cell lines from that of metastatic oral cell lines. We identified 9 genes that were commonly upregulated in nonmetastatic tongue cancer cell lines and downregulated in metastatic tongue carcinoma cell lines. 3 genes showed high degree in metastatic
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tongue cell lines and low degree in nonmetastatic tongue cell lined. The downregulation of 5 genes of them have been reported the relation to carcinoma and metastasis. We analyzed at the several times and got the same results. That suggested our cDNA microarray system was the useful. We analysed gene-expression profiles of the same scc cell lines using oligonucleotide microarray representing 54525 genes of Affymetrix chips. We identifed 206 genes that were upregulated and 434 genes downregulated in nonmetastatic tongue cancer cell lines, which were different expression in metrastatic scc cell lines. By comparison of cinicopathological parameters with the expression profiles, we are selecting the significant genes associated with metastatic abilities. These genome-wide expression profile should provide useful information for finding candidate genes whose products might serve as specific tumor markers and br as molecular targets for treatment of patients with oral scc, and establishing order-made treatment. Less
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